Metagenomics and marker gene approaches, coupled with high-throughput sequencing
technologies, have revolutionized the field of microbial ecology. Metagenomics is a culture …

Among available genome relatedness indices, average nucleotide identity (ANI) is one of
the most robust measurements of genomic relatedness between strains, and has great …

The 16S rRNA gene has been a mainstay of sequence-based bacterial analysis for
decades. However, high-throughput sequencing of the full gene has only recently become a …

Amplicon sequencing of tags such as 16S and ITS ribosomal RNA is a popular method for
investigating microbial populations. In such experiments, sequence errors caused by PCR …

Abstract Background The 16S rRNA gene is used extensively in bacterial phylogenetics, in
species delineation, and now widely in microbiome studies. However, the gene suffers from …

The role of 16S rRNA has been and largely remains crucial for the identification of microbial
organisms. Although 16S rRNA could certainly be described as one of the most studied …

Co-occurrence networks produced from microbial survey sequencing data are frequently
used to identify interactions between community members. While this approach has …

Climate change is having a dramatic impact on marine animal and plant communities but
little is known of its influence on marine prokaryotes, which represent the largest living …

Background Microbial communities (microbiota) influence human and animal disease and
immunity, geochemical nutrient cycling and plant productivity. Specific groups, including …

16S ribosomal RNA currently represents the most important target of study in bacterial
ecology. Its use for the description of bacterial diversity is, however, limited by the presence …