An optimized protocol for stepwise optimization of real-time RT-PCR analysis

F Zhao, NA Maren, PZ Kosentka, YY Liao… - Horticulture …, 2021 - academic.oup.com
Computational tool-assisted primer design for real-time reverse transcription (RT) PCR
(qPCR) analysis largely ignores the sequence similarities between sequences of …

[HTML][HTML] Reference Genes for Expression Analyses by qRT-PCR in Phthorimaea operculella (Lepidoptera: Gelechiidae)

CH Shen, LJ Peng, YX Zhang, HR Zeng, HF Yu, L **… - Insects, 2022 - mdpi.com
Simple Summary Quantitative real-time fluorescent polymerase chain reaction (qRT-PCR) is
a momentous tool for calculating the expression levels of targeted genes across various …

Development of Sarcophaga dux (diptera: Sarcophagidae) at constant temperatures and differential gene expression for age estimation of the pupae

X Zhang, Y Li, Y Shang, L Ren, W Chen, S Wang… - Journal of Thermal …, 2020 - Elsevier
Abstract Sarcophaga dux (Diptera: Sarcophagidae) is a necrophagous flesh fly species with
potential forensic value for estimating minimum postmortem interval (PMI min). The basic …

Evaluation of Reference Genes for Real-Time Quantitative PCR Analysis in Larvae of Spodoptera litura Exposed to Azadirachtin Stress Conditions

B Shu, J Zhang, G Cui, R Sun, V Sethuraman… - Frontiers in …, 2018 - frontiersin.org
Azadirachtin is an efficient and broad-spectrum botanical insecticide against more than 150
kinds of agricultural pests with the effects of mortality, antifeedant and growth regulation …

Differential Gene Expression for Age Estimation of Forensically Important Sarcophaga peregrina (Diptera: Sarcophagidae) Intrapuparial

Y Shang, L Ren, L Yang, S Wang… - Journal of medical …, 2020 - academic.oup.com
Sarcophaga peregrina is an important flesh fly species for estimating the minimum
postmortem interval (PMImin) in forensic entomology. The accurate determination of the …

Evaluation and Validation of Reference Genes for Quantitative Real-Time PCR in Helopeltis theivora Waterhouse (Hemiptera: Miridae)

Z Wang, Q Meng, X Zhu, S Sun, S Gao, Y Gou, A Liu - Scientific Reports, 2019 - nature.com
Helopeltis theivora Waterhouse is a predominant sucking pest in many tropic economic
crops, such as tea, cocoa and coffee. Quantitative real-time PCR (qRT-PCR) is one of the …

Evaluation of the expression stability of potential reference genes for RT-qPCR in Spodoptera frugipreda larvae exposed to camptothecin

Y Huang, X Yu, X ** genes for induced pluripotent stem cells and-derived endothelial cells for drug testing
SLM Ong, HJ Baelde, DGP van IJzendoorn… - Scientific Reports, 2022 - nature.com
There are no validated housekee** genes in induced pluripotent stem cells (iPSC) and
derived endothelial iPSC (iPSC-EC). Thus a comparison of gene expression levels is less …

Cyclic loading induces anabolic gene expression in ACLs in a load‐dependent and sex‐specific manner

L Paschall, S Carrozzi, E Tabdanov… - Journal of …, 2024 - Wiley Online Library
Anterior cruciate ligament (ACL) injuries are historically thought to be a result of a single
acute overload or traumatic event. However, recent studies suggest that ACL failure may be …

Evaluation of candidate reference genes for gene expression analysis in the brassica leaf beetle, Phaedon brassicae (Coleoptera: Chrysomelidae)

L Ma, T Jiang, X Liu, H **ao, Y Peng, W Zhang - PLoS One, 2021 - journals.plos.org
The brassica leaf beetle Phaedon brassicae is a notorious defoliator of cruciferous
vegetables. However, few molecular studies of this pest have been conducted due to limited …