Bioorthogonal chemistry is a set of methods using the chemistry of non-native functional
groups to explore and understand biology in living organisms. In this review, we summarize …

Protein-based conjugates are valuable constructs for a variety of applications. Conjugation
of proteins to fluorophores is commonly used to study their cellular localization and the …

Lysine (Lys) residues in proteins undergo a wide range of reversible post-translational
modifications (PTMs), which can regulate enzyme activities, chromatin structure, protein …

Reactions that occur under physiological conditions find diverse uses in the chemical and
biological sciences. However, the limitations that biological systems place on chemical …

Bioorthogonal phosphines were introduced in the context of the Staudinger ligation over 20
years ago. Since that time, phosphine probes have been used in myriad applications to tag …

The covalent attachment of Ub (ubiquitin) to target proteins (ubiquitylation) represents one of
the most versatile PTMs (post-translational modifications) in eukaryotic cells. Substrate …

Bioorthogonal chemistry comprises chemical reactions that can take place inside complex
biological environments, providing outstanding tools for the investigation and elucidation of …

Tetrasubstituted 1H‐imidazoles are a large family of heteroarene derivatives that are known
to include natural products isolated from marine sponges and synthetic compounds …

Going beyond the conventional approach of pairwise conjugation between two molecules,
the integration of multiple components onto a central scaffold molecule is essential for the …

A parallel, one-pot assembly approach to proteolysis targeting chimeras (PROTACs) is
demonstrated utilizing activated esters generated in situ, and traceless Staudinger ligation …