Strategies for sample labelling and library preparation in DNA metabarcoding studies

K Bohmann, V Elbrecht, C Carøe, I Bista… - Molecular Ecology …, 2022 - Wiley Online Library
Metabarcoding of DNA extracted from environmental or bulk specimen samples is
increasingly used to profile biota in basic and applied biodiversity research because of its …

Environmental DNA and environmental RNA: Current and prospective applications for biological monitoring

HD Veilleux, MD Misutka, CN Glover - Science of the Total Environment, 2021 - Elsevier
Traditional environmental biomonitoring approaches have limitations in terms of species
detectability and their capacity to account for spatial and temporal variation. Furthermore, as …

The future of DNA barcoding: Reflections from early career researchers

DM Grant, OB Brodnicke, AM Evankow, AO Ferreira… - Diversity, 2021 - mdpi.com
Over the last two decades, the use of DNA barcodes has transformed our ability to identify
and assess life on our planet. Both strengths and weaknesses of the method have been …

Integration of DNA-based approaches in aquatic ecological assessment using benthic macroinvertebrates

S Duarte, BR Leite, MJ Feio, FO Costa, AF Filipe - Water, 2021 - mdpi.com
Benthic macroinvertebrates are among the most used biological quality elements for
assessing the condition of all types of aquatic ecosystems worldwide (ie, fresh water …

[HTML][HTML] Targeted non-invasive bioindicator species detection in eDNA water samples to assess and monitor the integrity of vulnerable alpine freshwater environments

L Blattner, JN Ebner, J Zopfi, S von Fumetti - Ecological indicators, 2021 - Elsevier
Due to a high number of specialized species and unique environmental conditions, alpine
spring ecosystems are particularly vulnerable to environmental change and human impact …

Morphological vs. DNA metabarcoding approaches for the evaluation of stream ecological status with benthic invertebrates: Testing different combinations of markers …

A Meyer, F Boyer, A Valentini, A Bonin… - Molecular …, 2021 - Wiley Online Library
Macroinvertebrate assemblages are the most common bioindicators used for stream
biomonitoring, yet the standard approach exhibits several time‐consuming steps, including …

Comparing eDNA and Transect Methods for Aquatic Biodiversity Assessment in Lakes and Ponds

K Schwesig, V Zizka, C Scherber… - Molecular Ecology …, 2024 - Wiley Online Library
Biodiversity monitoring increasingly relies on molecular methods such as eDNA
metabarcoding. However, sound applications have so far been only established for a limited …

Pattern changes of microbial communities in urban river affected by anthropogenic activities and their environmental driving mechanisms

W Feng, J Gao, Y Wei, D Liu, F Yang, Q Zhang… - Environmental Sciences …, 2022 - Springer
The microbial community structure of sediments in the Bahe River Basin, China was studied
using a high-throughput sequencing platform and PCR amplification to investigate the …

Multi-group biodiversity distributions and drivers of metacommunity organization along a glacial–fluvial–limnic pathway on the Tibetan plateau

Q Lu, SY Zhang, J Du, Q Liu, C Dong, J Zhao… - Environmental …, 2023 - Elsevier
Extensive global glacial retreats are threatening cryosphere ecosystem functioning and the
associated biota in glacier-fed water systems. Understanding multi-group biodiversity …

Repeated subsamples during DNA extraction reveal increased diversity estimates in DNA metabarcoding of Malaise traps

VMA Zizka, MF Geiger, T Hörren, A Kirse… - Ecology and …, 2022 - Wiley Online Library
With increased application of DNA metabarcoding in biodiversity assessment, various
laboratory protocols have been optimized, and their further evaluation is subject of current …