[HTML][HTML] Genome engineering of the human gut microbiome

L Zheng, J Shen, R Chen, Y Hu, W Zhao… - Journal of Genetics and …, 2024 - Elsevier
The human gut microbiome, a complex ecosystem, significantly influences host health,
impacting crucial aspects such as metabolism and immunity. To enhance our …

Cold-tolerance mechanisms in foodborne pathogens: Escherichia coli and Listeria monocytogenes as examples

M Liu, Y Ding, Q Ye, S Wu, Q Gu, L Chen… - Critical Reviews in …, 2024 - Taylor & Francis
The cold chain is an integral part of the modern food industry. Low temperatures can
effectively alleviate food loss and the transmission of foodborne diseases caused by …

Metabolic engineering of Corynebacterium glutamicum for the high-level production of valerolactam, a nylon-5 monomer

T Han, SY Lee - Metabolic Engineering, 2023 - Elsevier
Valerolactam (VL) is an important precursor chemical for nylon-5 and nylon 6, 5. It has been
produced by petroleum-based route involving harsh reaction conditions and generating …

sRNA expression profile of KPC-2-producing carbapenem-resistant Klebsiella pneumoniae: Functional role of sRNA51

Y Bai, C **e, Y Zhang, Z Zhang, J Liu, G Cheng… - PLoS …, 2024 - journals.plos.org
The emergence of carbapenem-resistant Klebsiella pneumoniae (CRKP) has significant
challenges to human health and clinical treatment, with KPC-2-producing CRKP being the …

Metabolic engineering of Corynebacterium glutamicum for the production of pyrone and pyridine dicarboxylic acids

JS Cho, ZW Luo, CW Moon, CPS Prabowo… - Proceedings of the …, 2024 - pnas.org
Environmental concerns from plastic waste are driving interest in alternative monomers from
bio-based sources. Pseudoaromatic dicarboxylic acids are promising alternatives with …

[HTML][HTML] A library-based approach allows systematic and rapid evaluation of seed region length and reveals design rules for synthetic bacterial small RNAs

M Brück, TS Köbel, S Dittmar, AAR Rojas, J Georg… - Iscience, 2024 - cell.com
All organisms must respond to environmental changes. In bacteria, small RNAs (sRNAs) are
an important aspect of the regulation network underlying the adaptation to such changes …

Enhancing isoprenol production by systematically tuning metabolic pathways using CRISPR interference in E. coli

J Kim, TS Lee - Frontiers in Bioengineering and Biotechnology, 2023 - frontiersin.org
Regulation of metabolic gene expression is crucial for maximizing bioproduction titers.
Recent engineering tools including CRISPR/Cas9, CRISPR interference (CRISPRi), and …

CRISPR–dCas13a system for programmable small RNAs and polycistronic mRNA repression in bacteria

SC Ko, HM Woo - Nucleic Acids Research, 2024 - academic.oup.com
Bacterial small RNAs (sRNAs) function in post-transcriptional regulatory responses to
environmental changes. However, the lack of eukaryotic RNA interference-like machinery in …

Gene expression regulation by modulating Hfq expression in coordination with tailor-made sRNA-based knockdown in Escherichia coli

YJ Jung, KH Park, TY Jang, SM Yoo - Journal of Biotechnology, 2024 - Elsevier
The tailor-made synthetic sRNA-based gene expression knockdown system has
demonstrated its efficacy in achieving pathway balancing in microbes, facilitating precise …

Rewiring native post-transcriptional global regulators to achieve designer, multi-layered genetic circuits

TR Simmons, G Partipilo, R Buchser… - Nature …, 2024 - nature.com
As synthetic biology expands, creating “drag-and-drop” regulatory tools that can achieve
diverse regulatory outcomes are paramount. Herein, we develop a approach for engineering …