Is enzyme immobilization a mature discipline? Some critical considerations to capitalize on the benefits of immobilization

JM Bolivar, JM Woodley… - Chemical Society …, 2022 - pubs.rsc.org
Enzyme immobilization has been develo** since the 1960s and although many industrial
biocatalytic processes use the technology to improve enzyme performance, still today we …

[HTML][HTML] Stabilization of enzymes via immobilization: Multipoint covalent attachment and other stabilization strategies

RC Rodrigues, Á Berenguer-Murcia… - Biotechnology …, 2021 - Elsevier
The use of enzymes in industrial processes requires the improvement of their features in
many instances. Enzyme immobilization, a requirement to facilitate the recovery and reuse …

Use of Alcalase in the production of bioactive peptides: A review

VG Tacias-Pascacio, R Morellon-Sterling… - International journal of …, 2020 - Elsevier
This review aims to cover the uses of the commercially available protease Alcalase in the
production of biologically active peptides since 2010. Immobilization of Alcalase has also …

Parameters necessary to define an immobilized enzyme preparation

J Boudrant, JM Woodley, R Fernandez-Lafuente - Process Biochemistry, 2020 - Elsevier
Biocatalytic processes continue to find increasing application in industry. Therefore enzyme
immobilization has also become of increasing importance as a means of allowing enzyme …

Introducing a covalent thiol-based protected immobilized acetylcholinesterase with enhanced enzymatic performances for biosynthesis of esters

SRH Jangi, M Akhond - Process Biochemistry, 2022 - Elsevier
Acetylcholinesterase covalent immobilization was performed on mercaptoacetic acid-
functionalized magnetic nanoparticles. Upon the thiol-based protection of the enzyme active …

On the taught new tricks of enzymes immobilization: An all-inclusive overview

RA Wahab, N Elias, F Abdullah, SK Ghoshal - Reactive and Functional …, 2020 - Elsevier
The primary means of immobilizing enzymes are to boost the enzyme productivity and
operational stability, alongside facilitating the reuse of enzymes. Notwithstanding the …

[HTML][HTML] Immobilization of papain: A review

VG Tacias-Pascacio, R Morellon-Sterling… - International Journal of …, 2021 - Elsevier
Papain is a cysteine protease from papaya, with many applications due to its broad
specificity. This paper reviews for first time the immobilization of papain on different supports …

[HTML][HTML] β-Galactosidase from Kluyveromyces lactis: Characterization, production, immobilization and applications-A review

TL de Albuquerque, M de Sousa, NCG e Silva… - International Journal of …, 2021 - Elsevier
A review on the enzyme β-galactosidase from Kluyveromyces lactis is presented, from the
perspective of its structure and mechanisms of action, the main catalyzed reactions, the key …

[HTML][HTML] A review on the immobilization of pepsin: A Lys-poor enzyme that is unstable at alkaline pH values

R Morellon-Sterling, O Tavano, JM Bolivar… - International journal of …, 2022 - Elsevier
Pepsin is a protease used in many different applications, and in many instances, it is utilized
in an immobilized form to prevent contamination of the reaction product. This enzyme has …

Make proper surfaces for immobilization of enzymes: immobilization of lipase and α-amylase on modified Na-sepiolite

S Mortazavi, H Aghaei - International Journal of Biological Macromolecules, 2020 - Elsevier
This paper has focused on making suitable carriers for the immobilization of α-amylase from
Bacillus subtilis and lipase from Candida rugosa via adsorption on modified Na-sepiolite …