Forward genetic screens are powerful tools for the discovery and functional annotation of
genetic elements. Recently, the RNA-guided CRISPR (clustered regularly interspaced short …

Image-based screening is used to measure a variety of phenotypes in cells and whole
organisms. Combined with perturbations such as RNA interference, small molecules, and …

Forward genetic screens are powerful tools for the unbiased discovery and functional
characterization of specific genetic elements associated with a phenotype of interest …

Systematic interrogation of gene function requires the ability to perturb gene expression in a
robust and generalizable manner. Here we describe structure-guided engineering of a …

The simplicity of programming the CRISPR (clustered regularly interspaced short
palindromic repeats)–associated nuclease Cas9 to modify specific genomic loci suggests a …

Fruit flies of the genus Drosophila have been an attractive and effective genetic model
organism since Thomas Hunt Morgan and colleagues made seminal discoveries with them …

The identification of the Drosophila melanogaster Toll pathway cascade and the subsequent
characterization of TLRs have reshaped our understanding of the immune system. Ever …

▪ Abstract Tight control of cell-cell communication is essential for the generation of a
normally patterned embryo. A critical mediator of key cell-cell signaling events during …

Forward genetic screens in model organisms have provided important insights into
numerous aspects of development, physiology and pathology. With the availability of …

Antigen stimulation of immune cells triggers Ca2+ entry through Ca2+ release-activated
Ca2+ (CRAC) channels, promoting the immune response to pathogens by activating the …