Directed evolution aims to expedite the natural evolution process of biological molecules
and systems in a test tube through iterative rounds of gene diversifications and library …

Metabolic engineering aims to improve the production of economically valuable molecules
through the genetic manipulation of microbial metabolism. While the discipline is a little over …

Eukaryotic cells execute complex transcriptional programs in which specific loci throughout
the genome are regulated in distinct ways by targeted regulatory assemblies. We have …

Microbial bioproduction of chemicals, proteins, and primary metabolites from cheap carbon
sources is currently an advancing area in industrial research. The model yeast …

Global energy demand and environmental concerns have stimulated increasing efforts to
produce carbon-neutral fuels directly from renewable resources. Microbially derived …

Engineering cellular metabolism for improved production of valuable chemicals requires
extensive modulation of bacterial genome to explore complex genetic spaces. Here, we …

Metabolic engineering aims to develop efficient cell factories by rewiring cellular
metabolism. As one of the most commonly used cell factories, Saccharomyces cerevisiae …

Designing an optimal microbial cell factory often requires overexpression, knock-down, and
knock-out of multiple gene targets. Unfortunately, such rewiring of cellular metabolism is …

Yeasts have been used for thousands of years to make fermented foods and beverages,
such as beer, wine, sake, and bread. However, the choice for a particular yeast strain or …

Microbial fatty acid-derived fuels have emerged as promising alternatives to petroleum-
based transportation fuels. Here we report a modular engineering approach that …