Structural determinants and mechanism of HIV-1 genome packaging

K Lu, X Heng, MF Summers - Journal of molecular biology, 2011 - Elsevier
Like all retroviruses, the human immunodeficiency virus selectively packages two copies of
its unspliced RNA genome, both of which are utilized for strand-transfer-mediated …

How retroviruses select their genomes

V D'Souza, MF Summers - Nature Reviews Microbiology, 2005 - nature.com
As retroviruses assemble in infected cells, two copies of their full-length, unspliced RNA
genomes are selected for packaging from a cellular milieu that contains a substantial excess …

In vivo reconstitution finds multivalent RNA–RNA interactions as drivers of mesh-like condensates

W Ma, G Zhen, W **e, C Mayr - Elife, 2021 - elifesciences.org
Liquid-like condensates have been thought to be sphere-like. Recently, various
condensates with filamentous morphology have been observed in cells. One such …

High-throughput SHAPE analysis reveals structures in HIV-1 genomic RNA strongly conserved across distinct biological states

KA Wilkinson, RJ Gorelick, SM Vasa, N Guex… - PLoS …, 2008 - journals.plos.org
Replication and pathogenesis of the human immunodeficiency virus (HIV) is tightly linked to
the structure of its RNA genome, but genome structure in infectious virions is poorly …

NMR detection of structures in the HIV-1 5′-leader RNA that regulate genome packaging

K Lu, X Heng, L Garyu, S Monti, EL Garcia… - Science, 2011 - science.org
The 5′-leader of the HIV-1 genome regulates multiple functions during viral replication via
mechanisms that have yet to be established. We developed a nuclear magnetic resonance …

Trigger factor in complex with the ribosome forms a molecular cradle for nascent proteins

L Ferbitz, T Maier, H Patzelt, B Bukau, E Deuerling… - Nature, 2004 - nature.com
During protein biosynthesis, nascent polypeptide chains that emerge from the ribosomal exit
tunnel encounter ribosome-associated chaperones, which assist their folding to the native …

Structure of the HIV-1 RNA packaging signal

SC Keane, X Heng, K Lu, S Kharytonchyk… - Science, 2015 - science.org
The 5′ leader of the HIV-1 genome contains conserved elements that direct selective
packaging of the unspliced, dimeric viral RNA into assembling particles. By using a 2H …

Computationally reconstructing cotranscriptional RNA folding from experimental data reveals rearrangement of non-native folding intermediates

MY Angela, PM Gasper, L Cheng, LB Lai, S Kaur… - Molecular cell, 2021 - cell.com
The series of RNA folding events that occur during transcription can critically influence
cellular RNA function. Here, we present reconstructing RNA dynamics from data (R2D2), a …

The origin of genetic diversity in HIV-1

RP Smyth, MP Davenport, J Mak - Virus research, 2012 - Elsevier
One of the hallmarks of HIV infection is the rapid development of a genetically complex
population (quasispecies) from an initially limited number of infectious particles. Genetic …

Analysis of the initiating events in HIV-1 particle assembly and genome packaging

SB Kutluay, PD Bieniasz - PLoS pathogens, 2010 - journals.plos.org
HIV-1 Gag drives a number of events during the genesis of virions and is the only viral
protein required for the assembly of virus-like particles in vitro and in cells. Although a …