In vitro assembly, positioning and contraction of a division ring in minimal cells

S Kohyama, A Merino-Salomón, P Schwille - Nature Communications, 2022 - nature.com
Constructing a minimal machinery for autonomous self-division of synthetic cells is a major
goal of bottom-up synthetic biology. One paradigm has been the E. coli divisome, with the …

Phospholipid synthesis inside phospholipid membrane vesicles

S Eto, R Matsumura, Y Shimane, M Fujimi… - Communications …, 2022 - nature.com
Construction of living artificial cells from genes and molecules can expand our
understanding of life system and establish a new aspect of bioengineering. However, growth …

Nonequilibrium membrane dynamics induced by active protein interactions and chemical reactions: a review

H Noguchi - ChemSystemsChem, 2025 - Wiley Online Library
Biomembranes wrap** cells and organelles are not only the partitions that separate the
insides but also dynamic fields for biological functions accompanied by membrane shape …

Machine learning-aided design and screening of an emergent protein function in synthetic cells

S Kohyama, BP Frohn, L Babl, P Schwille - Nature Communications, 2024 - nature.com
Abstract Recently, utilization of Machine Learning (ML) has led to astonishing progress in
computational protein design, bringing into reach the targeted engineering of proteins for …

Engineering spatiotemporal organization and dynamics in synthetic cells

A Groaz, H Moghimianavval, F Tavella… - Wiley …, 2021 - Wiley Online Library
Constructing synthetic cells has recently become an appealing area of research. Decades of
research in biochemistry and cell biology have amassed detailed part lists of components …

Phase-space geometry of mass-conserving reaction-diffusion dynamics

F Brauns, J Halatek, E Frey - Physical Review X, 2020 - APS
Experimental studies of protein-pattern formation have stimulated new interest in the
dynamics of reaction-diffusion systems. However, a comprehensive theoretical …

Transcription and translation in cytomimetic protocells perform most efficiently at distinct macromolecular crowding conditions

MA Vibhute, MH Schaap, RJM Maas… - ACS Synthetic …, 2020 - ACS Publications
The formation of cytomimetic protocells that capture the physicochemical aspects of living
cells is an important goal in bottom-up synthetic biology. Here, we recreated the crowded …

Continuous cell-free replication and evolution of artificial genomic DNA in a compartmentalized gene expression system

H Okauchi, N Ichihashi - ACS Synthetic Biology, 2021 - ACS Publications
In all living organisms, genomic DNA continuously replicates by the proteins encoded in
itself and undergoes evolution through many generations of replication. This continuous …

Time-reversal and parity-time symmetry breaking in non-Hermitian field theories

T Suchanek, K Kroy, SAM Loos - Physical Review E, 2023 - APS
We study time-reversal symmetry breaking in non-Hermitian fluctuating field theories with
conserved dynamics, comprising the mesoscopic descriptions of a wide range of …

Bulk-surface coupling identifies the mechanistic connection between Min-protein patterns in vivo and in vitro

F Brauns, G Pawlik, J Halatek, J Kerssemakers… - Nature …, 2021 - nature.com
Self-organisation of Min proteins is responsible for the spatial control of cell division in
Escherichia coli, and has been studied both in vivo and in vitro. Intriguingly, the protein …