Recent advances in cryo-electron microscopy have marked only the beginning of the
potential of this technique. To bring structure into cell biology, the modality of cryo-electron …

The recent proliferation of cryo-electron tomography (cryo-ET) techniques has led to the cryo-
ET resolution revolution. Meanwhile, significant efforts have been made to improve the …

A defining pathological feature of most neurodegenerative diseases is the assembly of
proteins into amyloid that form disease-specific structures. In Alzheimer's disease, this is …

Cryo-electron tomograms capture a wealth of structural information on the molecular
constituents of cells and tissues. We present DeePiCt (deep picker in context), an open …

The structure of chromatin plays pivotal roles in regulating gene transcription, DNA
replication and repair, and chromosome segregation. This structure, however, remains …

Cryogenic-electron tomography enables the visualization of cellular environments in
extreme detail, however, tools to analyze the full amount of information contained within …

Cell-intrinsic defences constitute the first line of defence against intracellular pathogens. The
guanosine triphosphatase RAB32 orchestrates one such defence response against the …

Electron tomography of frozen, hydrated samples allows structure determination of
macromolecular complexes that are embedded in complex environments. Provided that the …

Systemic light chain (LC) amyloidosis (AL) is a disease where organs are damaged by an
overload of a misfolded patient-specific antibody-derived LC, secreted by an abnormal B cell …

Structures of macromolecules in their native state provide unique unambiguous insights into
their functions. Cryo-electron tomography combined with subtomogram averaging …