Signal Peptide-Dependent Protein Transport inBacillus subtilis: a Genome-Based Survey of the Secretome

H Tjalsma, A Bolhuis, JDH Jongbloed… - Microbiology and …, 2000 - journals.asm.org
One of the most salient features of Bacillus subtilis and related bacilli is their natural capacity
to secrete a variety of proteins into their environment, frequently to high concentrations. This …

Bacillus protein secretion: an unfolding story

CR Harwood, R Cranenburgh - Trends in microbiology, 2008 - cell.com
Bacillus subtilis and its close relatives are widely used for the production of enzymes for the
detergent, food and beverage industries. These organisms not only produce an appropriate …

Translocation of proteins across the cell envelope of Gram-positive bacteria

KHM van Wely, J Swaving, R Freudl… - FEMS microbiology …, 2001 - academic.oup.com
In contrast to Gram-negative bacteria, secretory proteins of Gram-positive bacteria only need
to traverse a single membrane to enter the extracellular environment. For this reason, Gram …

Re-annotating the Mycoplasma pneumoniae genome sequence: adding value, function and reading frames

T Dandekar, M Huynen, JT Regula… - Nucleic acids …, 2000 - academic.oup.com
Four years after the original sequence submission, we have re-annotated the genome of
Mycoplasma pneumoniae to incorporate novel data. The total number of ORFss has been …

Escherichia coli translocase: the unravelling of a molecular machine

EH Manting, AJM Driessen - Molecular microbiology, 2000 - Wiley Online Library
Protein translocation across the bacterial cytoplasmic membrane has been studied
extensively in Escherichia coli. The identification of the components involved and …

Towards the development of Bacillus subtilis as a cell factory for membrane proteins and protein complexes

JC Zweers, I Barák, D Becher, AJM Driessen… - Microbial cell …, 2008 - Springer
Abstract Background The Gram-positive bacterium Bacillus subtilis is an important producer
of high quality industrial enzymes and a few eukaryotic proteins. Most of these proteins are …

Proteome analysis of Bacillus subtilis extracellular proteins: a two-dimensional protein electrophoretic study

I Hirose, K Sano, I Shioda, M Kumano… - …, 2000 - microbiologyresearch.org
To analyse the proteome of Bacillus subtilis extracellular proteins, extracellular protein
samples were prepared from culture media (minimal medium containing 0· 4% glucose) of …

[HTML][HTML] The protein-conducting channel SecYEG

AKJ Veenendaal, C van der Does… - Biochimica et Biophysica …, 2004 - Elsevier
In bacteria, the translocase mediates the translocation of proteins into or across the cytosolic
membrane. It consists of a membrane embedded protein-conducting channel and a …

[HTML][HTML] SecYEG assembles into a tetramer to form the active protein translocation channel

EH Manting, C van der Does, H Remigy, A Engel… - The EMBO …, 2000 - embopress.org
Translocase mediates preprotein translocation across the Escherichia coli inner membrane.
It consists of the SecYEG integral membrane protein complex and the peripheral ATPase …

Heterologous protein secretion by Bacillus species: from the cradle to the grave

S Pohl, CR Harwood - Advances in applied microbiology, 2010 - Elsevier
Abstract The Gram-positive bacterium Bacillus subtilis and some of its close relatives are
widely used for the industrial production of enzymes for the detergents, food, and beverage …