Low-affinity binding sites and the transcription factor specificity paradox in eukaryotes
Eukaryotic transcription factors (TFs) from the same structural family tend to bind similar DNA
sequences, despite the ability of these TFs to execute distinct functions in vivo. The cell …
sequences, despite the ability of these TFs to execute distinct functions in vivo. The cell …
An efficient targeted nuclease strategy for high-resolution map** of DNA binding sites
We describe Cleavage Under Targets and Release Using Nuclease (CUT&RUN), a
chromatin profiling strategy in which antibody-targeted controlled cleavage by micrococcal …
chromatin profiling strategy in which antibody-targeted controlled cleavage by micrococcal …
The GAL4 system: a versatile system for the manipulation and analysis of gene expression
Since its introduction in 1993, the GAL4 system has become an essential part of the
Drosophila geneticist's toolkit. Widely used to drive gene expression in a multitude of cell …
Drosophila geneticist's toolkit. Widely used to drive gene expression in a multitude of cell …
[HTML][HTML] Organoid modeling of human fetal lung alveolar development reveals mechanisms of cell fate patterning and neonatal respiratory disease
Variation in lung alveolar development is strongly linked to disease susceptibility. However,
underlying cellular and molecular mechanisms are difficult to study in humans. We have …
underlying cellular and molecular mechanisms are difficult to study in humans. We have …
A complete temporal transcription factor series in the fly visual system
The brain consists of thousands of neuronal types that are generated by stem cells
producing different neuronal types as they age. In Drosophila, this temporal patterning is …
producing different neuronal types as they age. In Drosophila, this temporal patterning is …
Decoding gene regulation in the fly brain
The Drosophila brain is a frequently used model in neuroscience. Single-cell transcriptome
analysis,,,,–, three-dimensional morphological classification and electron microscopy …
analysis,,,,–, three-dimensional morphological classification and electron microscopy …
[HTML][HTML] TRIBE: hijacking an RNA-editing enzyme to identify cell-specific targets of RNA-binding proteins
RNA transcripts are bound and regulated by RNA-binding proteins (RBPs). Current methods
for identifying in vivo targets of an RBP are imperfect and not amenable to examining small …
for identifying in vivo targets of an RBP are imperfect and not amenable to examining small …
A large-scale resource for tissue-specific CRISPR mutagenesis in Drosophila
Genetic screens are powerful tools for the functional annotation of genomes. In the context of
multicellular organisms, interrogation of gene function is greatly facilitated by methods that …
multicellular organisms, interrogation of gene function is greatly facilitated by methods that …
Cell cycle heterogeneity directs the timing of neural stem cell activation from quiescence
Quiescent stem cells in adult tissues can be activated for homeostasis or repair. Neural stem
cells (NSCs) in Drosophila are reactivated from quiescence in response to nutrition by the …
cells (NSCs) in Drosophila are reactivated from quiescence in response to nutrition by the …
Cell cycle dynamics of lamina‐associated DNA
T van Schaik, M Vos, D Peric‐Hupkes, P HN Celie… - EMBO …, 2020 - embopress.org
In mammalian interphase nuclei, more than one thousand large genomic regions are
positioned at the nuclear lamina (NL). These lamina‐associated domains (LAD s) are …
positioned at the nuclear lamina (NL). These lamina‐associated domains (LAD s) are …