Technical pitfalls when collecting, cryopreserving, thawing, and stimulating human T-cells
The collection, cryopreservation, thawing, and culture of peripheral blood mononuclear cells
(PBMCs) can profoundly influence T cell viability and immunogenicity. Gold-standard PBMC …
(PBMCs) can profoundly influence T cell viability and immunogenicity. Gold-standard PBMC …
Extracellular flux analyses reveal differences in mitochondrial PBMC metabolism between high-fit and low-fit females
JJE Janssen, B Lagerwaard… - American Journal …, 2022 - journals.physiology.org
Analyzing metabolism of peripheral blood mononuclear cells (PBMCs) can possibly serve
as a cellular metabolic read-out for lifestyle factors and lifestyle interventions. However, the …
as a cellular metabolic read-out for lifestyle factors and lifestyle interventions. However, the …
A PD-L1xCD3 bispecific nanobody as a novel T-cell engager in treating PD-L1 overexpression melanoma
B Li, S Wang, B Shan, B Li, F Li - Molecular Immunology, 2023 - Elsevier
The development of Immune checkpoint blockade (ICB) therapy and BRAF-and MEK-
targeted therapies has reshaped the survival outcomes of the patients with advanced …
targeted therapies has reshaped the survival outcomes of the patients with advanced …
[HTML][HTML] Guidelines for analysis of low-frequency antigen-specific T cell results: Dye-based proliferation assay vs 3H-thymidine incorporation
D Di Blasi, I Claessen, AW Turksma, J van Beek… - Journal of …, 2020 - Elsevier
It is generally recognized that dysregulation of the immune system plays a critical role in
many diseases, including autoimmune diseases and cancer. T cells play a crucial role in …
many diseases, including autoimmune diseases and cancer. T cells play a crucial role in …
Identification of a PD1/PD‐L1 inhibitor by structure‐based pharmacophore modelling, virtual screening, molecular docking and biological evaluation
Abstract PD‐1/PD‐L1 is a critical druggable target for immunotherapy against sepsis.
Chemoinformatics techniques involved the structure‐based 3D pharmacophore model …
Chemoinformatics techniques involved the structure‐based 3D pharmacophore model …
Supplementation with vitamin E, Zinc, Selenium, and copper re-establishes T-cell function and improves motor recovery in a rat model of spinal cord injury
E Garcia, F Hernández-Ayvar… - Cell …, 2022 - journals.sagepub.com
Spinal cord injury (SCI) causes a dysfunction of sympathetic nervous system innervation that
affects the immune system, leading to immunosuppression syndrome (ISS) and contributing …
affects the immune system, leading to immunosuppression syndrome (ISS) and contributing …
Boswellia carterii oleoresin extracts induce caspase-mediated apoptosis and G1 cell cycle arrest in human leukaemia subtypes
Background: Leukemias are a common cancer in adults and children. While existing
treatments are effective, they are associated with severe side-effects compounded by the …
treatments are effective, they are associated with severe side-effects compounded by the …
[HTML][HTML] Standard peripheral blood mononuclear cell cryopreservation selectively decreases detection of nine clinically relevant T cell markers
CM Capelle, S Ciré, W Ammerlaan… - …, 2021 - journals.aai.org
Biobanking is an operational component of various epidemiological studies and clinical
trials. Although peripheral blood is routinely acquired and stored in biobanks, the effects of …
trials. Although peripheral blood is routinely acquired and stored in biobanks, the effects of …
Peripheral blood mononuclear cell mitochondrial dysfunction in acute alcohol‐associated hepatitis
Background Patients with acute alcohol‐associated hepatitis (AH) have immune
dysfunction. Mitochondrial function is critical for immune cell responses and regulates …
dysfunction. Mitochondrial function is critical for immune cell responses and regulates …
Comprehensive evaluation of the effects of long-term cryopreservation on peripheral blood mononuclear cells using flow cytometry
B Li, C Yang, G Jia, Y Liu, N Wang, F Yang, R Su… - BMC immunology, 2022 - Springer
Human peripheral blood mononuclear cells (PBMCs) originate from hematopoietic stem
cells in the bone marrow, which mainly includes lymphocytes (T cells, B cells, and natural …
cells in the bone marrow, which mainly includes lymphocytes (T cells, B cells, and natural …