Light sheet fluorescence microscopy

EHK Stelzer, F Strobl, BJ Chang, F Preusser… - Nature Reviews …, 2021 - nature.com
Light sheet fluorescence microscopy (LSFM) uses a thin sheet of light to excite only
fluorophores within the focal volume. Light sheet microscopes (LSMs) have a true optical …

Zebrafish as an animal model for biomedical research

TY Choi, TI Choi, YR Lee, SK Choe… - Experimental & Molecular …, 2021 - nature.com
Zebrafish have several advantages compared to other vertebrate models used in modeling
human diseases, particularly for large-scale genetic mutant and therapeutic compound …

Rationalized deep learning super-resolution microscopy for sustained live imaging of rapid subcellular processes

C Qiao, D Li, Y Liu, S Zhang, K Liu, C Liu, Y Guo… - Nature …, 2023 - nature.com
The goal when imaging bioprocesses with optical microscopy is to acquire the most
spatiotemporal information with the least invasiveness. Deep neural networks have …

Adaptive optics for high-resolution imaging

KM Hampson, R Turcotte, DT Miller… - Nature Reviews …, 2021 - nature.com
Adaptive optics (AO) is a technique that corrects for optical aberrations. It was originally
proposed to correct for the blurring effect of atmospheric turbulence on images in ground …

Da** metastasis

G Follain, D Herrmann, S Harlepp, V Hyenne… - Nature Reviews …, 2020 - nature.com
Metastasis is a dynamic succession of events involving the dissemination of tumour cells to
distant sites within the body, ultimately reducing the survival of patients with cancer. To …

Anti-quenching NIR-II molecular fluorophores for in vivo high-contrast imaging and pH sensing

S Wang, Y Fan, D Li, C Sun, Z Lei, L Lu, T Wang… - Nature …, 2019 - nature.com
The contrast and sensitivity of in vivo fluorescence imaging has been revolutionized by
molecular fluorophores operating in the second near-infrared window (NIR-II; 1000-1700 …

Applications, promises, and pitfalls of deep learning for fluorescence image reconstruction

C Belthangady, LA Royer - Nature methods, 2019 - nature.com
Deep learning is becoming an increasingly important tool for image reconstruction in
fluorescence microscopy. We review state-of-the-art applications such as image restoration …

In vivo NIR-II fluorescence imaging for biology and medicine

F Wang, Y Zhong, O Bruns, Y Liang, H Dai - Nature Photonics, 2024 - nature.com
Owing to reduced light scattering and tissue autofluorescence, in vivo fluorescence imaging
in the 1,000–3,000-nm near-infrared II (NIR-II) spectral range can afford non-invasive …

Iterative tomography with digital adaptive optics permits hour-long intravital observation of 3D subcellular dynamics at millisecond scale

J Wu, Z Lu, D Jiang, Y Guo, H Qiao, Y Zhang, T Zhu… - Cell, 2021 - cell.com
Long-term subcellular intravital imaging in mammals is vital to study diverse intercellular
behaviors and organelle functions during native physiological processes. However, optical …