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A guide to membrane protein X‐ray crystallography
Membrane proteins play critical physiological roles in all organisms, from ion transport and
signal transduction to multidrug resistance. Elucidating their 3D structures is essential for …
signal transduction to multidrug resistance. Elucidating their 3D structures is essential for …
An overview of the top ten detergents used for membrane protein crystallization
To study integral membrane proteins, one has to extract them from the membrane—the step
that is typically achieved by the application of detergents. In this mini-review, we summarize …
that is typically achieved by the application of detergents. In this mini-review, we summarize …
A general protocol for the generation of Nanobodies for structural biology
There is growing interest in using antibodies as auxiliary tools to crystallize proteins. Here
we describe a general protocol for the generation of Nanobodies to be used as …
we describe a general protocol for the generation of Nanobodies to be used as …
Membrane proteins bind lipids selectively to modulate their structure and function
Previous studies have established that the folding, structure and function of membrane
proteins are influenced by their lipid environments,,,,,, and that lipids can bind to specific …
proteins are influenced by their lipid environments,,,,,, and that lipids can bind to specific …
Mass spectrometry of intact membrane protein complexes
Mass spectrometry (MS) of intact soluble protein complexes has emerged as a powerful
technique to study the stoichiometry, structure-function and dynamics of protein assemblies …
technique to study the stoichiometry, structure-function and dynamics of protein assemblies …
Lipid–protein interactions in plasma membrane organization and function
Lipid–protein interactions in cells are involved in various biological processes, including
metabolism, trafficking, signaling, host–pathogen interactions, and transmembrane …
metabolism, trafficking, signaling, host–pathogen interactions, and transmembrane …
High-throughput stability screening for detergent-solubilized membrane proteins
Protein stability in detergent or membrane-like environments is the bottleneck for structural
studies on integral membrane proteins (IMP). Irrespective of the method to study the …
studies on integral membrane proteins (IMP). Irrespective of the method to study the …
Denaturing and native mass spectrometric analytics for biotherapeutic drug discovery research: historical, current, and future personal perspectives
IDG Campuzano, W Sandoval - … of the American Society for Mass …, 2021 - ACS Publications
Mass spectrometry (MS) plays a key role throughout all stages of drug development and is
now as ubiquitous as other analytical techniques such as surface plasmon resonance …
now as ubiquitous as other analytical techniques such as surface plasmon resonance …
Porous nucleating agents for protein crystallization
Solving the structure of proteins is pivotal to achieving success in rational drug design and in
other biotechnological endeavors. The most powerful method for determining the structure of …
other biotechnological endeavors. The most powerful method for determining the structure of …
Nanoliter-scale protein crystallization and screening with a microfluidic droplet robot
Large-scale screening of hundreds or even thousands of crystallization conditions while with
low sample consumption is in urgent need, in current structural biology research. Here we …
low sample consumption is in urgent need, in current structural biology research. Here we …