New insights into the formation and the function of lamellipodia and ruffles in mesenchymal cell migration

M Innocenti - Cell Adhesion & Migration, 2018 - Taylor & Francis
Lamellipodia and ruffles are veil-shaped cell protrusions composed of a highly branched
actin filament meshwork assembled by the Arp2/3 complex. These structures not only …

About samples, giving examples: optimized single molecule localization microscopy

A Jimenez, K Friedl, C Leterrier - Methods, 2020 - Elsevier
Super-resolution microscopy has profoundly transformed how we study the architecture of
cells, revealing unknown structures and refining our view of cellular assemblies. Among the …

Dynamin regulates the dynamics and mechanical strength of the actin cytoskeleton as a multifilament actin-bundling protein

R Zhang, DM Lee, JR Jimah, N Gerassimov… - Nature cell …, 2020 - nature.com
The dynamin GTPase is known to bundle actin filaments, but the underlying molecular
mechanism and physiological relevance remain unclear. Our genetic analyses revealed a …

4polar-STORM polarized super-resolution imaging of actin filament organization in cells

CV Rimoli, CA Valades-Cruz, V Curcio… - Nature …, 2022 - nature.com
Single-molecule localization microscopy provides insights into the nanometer-scale spatial
organization of proteins in cells, however it does not provide information on their …

Flat clathrin lattices are dynamic actin-controlled hubs for clathrin-mediated endocytosis and signalling of specific receptors

D Leyton-Puig, T Isogai, E Argenzio… - Nature …, 2017 - nature.com
Clathrin lattices at the plasma membrane coat both invaginated and flat regions forming
clathrin-coated pits and clathrin plaques, respectively. The function and regulation of clathrin …

Automating multimodal microscopy with NanoJ-Fluidics

P Almada, PM Pereira, S Culley, G Caillol… - Nature …, 2019 - nature.com
Combining and multiplexing microscopy approaches is crucial to understand cellular events,
but requires elaborate workflows. Here, we present a robust, open-source approach for …

mTORC1 and mTORC2 converge on the Arp2/3 complex to promote KrasG12D-induced acinar-to-ductal metaplasia and early pancreatic carcinogenesis

Y Zhao, B Schoeps, D Yao, Z Zhang, K Schuck… - Gastroenterology, 2021 - Elsevier
Background & Aims Oncogenic Kras G12D induces neoplastic transformation of pancreatic
acinar cells through acinar-to-ductal metaplasia (ADM), an actin-based morphogenetic …

Super-resolved visualization of single DNA-based tension sensors in cell adhesion

T Schlichthaerle, C Lindner, R Jungmann - Nature Communications, 2021 - nature.com
Cell-extracellular matrix sensing plays a crucial role in cellular behavior and leads to the
formation of a macromolecular protein complex called the focal adhesion. Despite their …

Fix your membrane receptor imaging: actin cytoskeleton and CD4 membrane organization disruption by chemical fixation

PM Pereira, D Albrecht, S Culley, C Jacobs… - Frontiers in …, 2019 - frontiersin.org
Single-molecule localization microscopy (SMLM) techniques allow near molecular scale
resolution (~ 20 nm) as well as precise and robust analysis of protein organization at …

[HTML][HTML] Investigation of red blood cells by atomic force microscopy

V Sergunova, S Leesment, A Kozlov, V Inozemtsev… - Sensors, 2022 - mdpi.com
Currently, much research is devoted to the study of biological objects using atomic force
microscopy (AFM). This method's resolution is superior to the other non-scanning …