From purines to purinergic signalling: molecular functions and human diseases

Z Huang, N **e, P Illes, F Di Virgilio, H Ulrich… - … and Targeted Therapy, 2021 - nature.com
Purines and their derivatives, most notably adenosine and ATP, are the key molecules
controlling intracellular energy homoeostasis and nucleotide synthesis. Besides, these …

Targeting nucleotide metabolism: a promising approach to enhance cancer immunotherapy

H Wu, Y Gong, P Ji, Y **e, YZ Jiang, G Liu - Journal of Hematology & …, 2022 - Springer
Targeting nucleotide metabolism can not only inhibit tumor initiation and progression but
also exert serious side effects. With in-depth studies of nucleotide metabolism, our …

A new view into the regulation of purine metabolism: the purinosome

AM Pedley, SJ Benkovic - Trends in biochemical sciences, 2017 - cell.com
Other than serving as building blocks for DNA and RNA, purine metabolites provide a cell
with the necessary energy and cofactors to promote cell survival and proliferation. A …

Potential mechanisms connecting purine metabolism and cancer therapy

J Yin, W Ren, X Huang, J Deng, T Li, Y Yin - Frontiers in immunology, 2018 - frontiersin.org
Unrestricted cell proliferation is a hallmark of cancer. Purines are basic components of
nucleotides in cell proliferation, thus impaired purine metabolism is associated with the …

The role of dynamic enzyme assemblies and substrate channelling in metabolic regulation

LJ Sweetlove, AR Fernie - Nature communications, 2018 - nature.com
Transient physical association between enzymes appears to be a cardinal feature of
metabolic systems, yet the purpose of this metabolic organisation remains enigmatic. It is …

Metabolomics and mass spectrometry imaging reveal channeled de novo purine synthesis in cells

V Pareek, H Tian, N Winograd, SJ Benkovic - Science, 2020 - science.org
Metabolons, multiprotein complexes consisting of sequential enzymes of a metabolic
pathway, are proposed to be biosynthetic “hotspots” within the cell. However, experimental …

The role of liquid–liquid phase separation in regulating enzyme activity

BG O'Flynn, T Mittag - Current opinion in cell biology, 2021 - Elsevier
Liquid–liquid phase separation (LLPS) is now recognized as a common mechanism
underlying regulation of enzyme activity in cells. Insights from studies in cells are …

Phase separation of TPX2 enhances and spatially coordinates microtubule nucleation

MR King, S Petry - Nature communications, 2020 - nature.com
Phase separation of substrates and effectors is proposed to enhance biological reaction
rates and efficiency. Targeting protein for Xklp2 (TPX2) is an effector of branching …

Human de novo purine biosynthesis

V Pareek, AM Pedley, SJ Benkovic - Critical reviews in …, 2021 - Taylor & Francis
The focus of this review is the human de novo purine biosynthetic pathway. The pathway
enzymes are enumerated, as well as the reactions they catalyze and their physical …

Spatial colocalization and functional link of purinosomes with mitochondria

JB French, SA Jones, H Deng, AM Pedley, D Kim… - Science, 2016 - science.org
Purine biosynthetic enzymes organize into dynamic cellular bodies called purinosomes.
Little is known about the spatiotemporal control of these structures. Using super-resolution …