Detection technologies for RNA modifications

Y Zhang, L Lu, X Li - Experimental & molecular medicine, 2022 - nature.com
To date, more than 170 chemical modifications have been characterized in RNA, providing a
new layer of gene expression regulation termed the 'epitranscriptome'. RNA modification …

[HTML][HTML] Epitranscriptome: review of top 25 most-studied RNA modifications

VA Arzumanian, GV Dolgalev, IY Kurbatov… - International Journal of …, 2022 - mdpi.com
The alphabet of building blocks for RNA molecules is much larger than the standard four
nucleotides. The diversity is achieved by the post-transcriptional biochemical modification of …

Semi-quantitative detection of pseudouridine modifications and type I/II hypermodifications in human mRNAs using direct long-read sequencing

S Tavakoli, M Nabizadeh, A Makhamreh… - Nature …, 2023 - nature.com
Here, we develop and apply a semi-quantitative method for the high-confidence
identification of pseudouridylated sites on mammalian mRNAs via direct long-read …

RMDisease V2. 0: an updated database of genetic variants that affect RNA modifications with disease and trait implication

B Song, X Wang, Z Liang, J Ma, D Huang… - Nucleic Acids …, 2023 - academic.oup.com
Recent advances in epitranscriptomics have unveiled functional associations between RNA
modifications (RMs) and multiple human diseases, but distinguishing the functional or …

Quantification and map** of RNA modifications

J ** reveals a diverse dihydrouridine landscape including mRNA
AS Draycott, C Schaening-Burgos… - PLoS …, 2022 - journals.plos.org
Dihydrouridine is a modified nucleotide universally present in tRNAs, but the complete
dihydrouridine landscape is unknown in any organism. We introduce dihydrouridine …

Dysregulation of RNA modification systems in clinical populations with neurocognitive disorders

HM Knight, MD Öz… - Neural Regeneration …, 2024 - journals.lww.com
The study of modified RNA known as epitranscriptomics has become increasingly relevant
in our understanding of disease-modifying mechanisms. Methylation of N6 adenosine (m 6 …

All the sites we cannot see: Sources and mitigation of false negatives in RNA modification studies

S Oberdoeffer, WV Gilbert - Nature Reviews Molecular Cell Biology, 2024 - nature.com
RNA modifications are essential for human health—too much or too little of them leads to
serious illnesses ranging from neurodevelopmental disorders to cancer. Technical …

Functional redundancy in tRNA dihydrouridylation

C Sudol, LM Kilz, V Marchand, Q Thullier… - Nucleic Acids …, 2024 - academic.oup.com
Dihydrouridine (D) is a common modified base found predominantly in transfer RNA (tRNA).
Despite its prevalence, the mechanisms underlying dihydrouridine biosynthesis, particularly …