The human gene catalogue is essentially complete, but we lack an equivalently vetted
inventory of bona fide human enhancers. Hundreds of thousands of candidate enhancers …

Shadow enhancers are seemingly redundant transcriptional cis-regulatory elements that
regulate the same gene and drive overlap** expression patterns. Recent studies have …

Most variants associated with complex traits and diseases identified by genome-wide
association studies (GWAS) map to noncoding regions of the genome with unknown effects …

A gene can be defined as essential when loss of its function compromises viability of the
individual (for example, embryonic lethality) or results in profound loss of fitness. At the …

CRISPR/Cas9-mediated cleavage of DNA, which depends on the endonuclease activity of
Cas9, has been widely used for gene editing due to its excellent programmability and …

Map** cis-acting expression quantitative trait loci (cis-eQTL) has become a popular
approach for characterizing proximal genetic regulatory variants. In this paper, we describe …

CRISPR screen technology enables systematic and scalable interrogation of gene function
by using the CRISPR-Cas9 system to perturb gene expression. In the field of cancer …

To interrogate genes essential for cell growth, proliferation and survival in human cells, we
carried out a genome-wide clustered regularly interspaced short palindromic repeat …

The precise spatiotemporal gene expression is orchestrated by enhancers that lack general
sequence features and thus are difficult to be computationally identified. By nascent RNA …

Various exogeneous and endogenous factors constantly cause damages in the
biomolecules within a cell. For example, per day, 10,000–100,000 molecular lesions occur …