Nanopore-based technologies beyond DNA sequencing

YL Ying, ZL Hu, S Zhang, Y Qing, A Fragasso… - Nature …, 2022 - nature.com
Inspired by the biological processes of molecular recognition and transportation across
membranes, nanopore techniques have evolved in recent decades as ultrasensitive …

Nanopore DNA sequencing technologies and their applications towards single-molecule proteomics

A Dorey, S Howorka - Nature chemistry, 2024 - nature.com
Sequencing of nucleic acids with nanopores has emerged as a powerful tool offering rapid
readout, high accuracy, low cost and portability. This label-free method for sequencing at the …

Solid-state nanopore sensors

L Xue, H Yamazaki, R Ren, M Wanunu… - Nature Reviews …, 2020 - nature.com
Nanopore-based sensors have established themselves as a prominent tool for solution-
based, single-molecule analysis of the key building blocks of life, including nucleic acids …

Nanopore sequencing of DNA-barcoded probes for highly multiplexed detection of microRNA, proteins and small biomarkers

C Koch, B Reilly-O'Donnell, R Gutierrez… - Nature …, 2023 - nature.com
There is an unmet need to develop low-cost, rapid and highly multiplexed diagnostic
technology platforms for quantitatively detecting blood biomarkers to advance clinical …

Single-molecule amplification-free multiplexed detection of circulating microRNA cancer biomarkers from serum

S Cai, T Pataillot-Meakin, A Shibakawa, R Ren… - Nature …, 2021 - nature.com
MicroRNAs (miRNAs) play essential roles in post-transcriptional gene expression and are
also found freely circulating in bodily fluids such as blood. Dysregulated miRNA signatures …

Multiplexed detection of viral antigen and RNA using nanopore sensing and encoded molecular probes

R Ren, S Cai, X Fang, X Wang, Z Zhang… - Nature …, 2023 - nature.com
We report on single-molecule nanopore sensing combined with position-encoded DNA
molecular probes, with chemistry tuned to simultaneously identify various antigen proteins …

Identification of single amino acid differences in uniformly charged homopolymeric peptides with aerolysin nanopore

F Piguet, H Ouldali, M Pastoriza-Gallego… - Nature …, 2018 - nature.com
There are still unmet needs in finding new technologies for biomedical diagnostic and
industrial applications. A technology allowing the analysis of size and sequence of short …

In situ solid-state nanopore fabrication

JP Fried, JL Swett, BP Nadappuram, JA Mol… - Chemical Society …, 2021 - pubs.rsc.org
Nanopores in solid-state membranes are promising for a wide range of applications
including DNA sequencing, ultra-dilute analyte detection, protein analysis, and polymer data …

Confined nanopipette sensing: from single molecules, single nanoparticles, to single cells

RJ Yu, YL Ying, R Gao, YT Long - … Chemie International Edition, 2019 - Wiley Online Library
Nanopipettes provide a promising confined space that enables advances in
electrochemical, optical, and mass spectrometric measurements at the nanoscale. They …

The application of single molecule nanopore sensing for quantitative analysis

Y Wu, JJ Gooding - Chemical Society Reviews, 2022 - pubs.rsc.org
Nanopore-based sensors typically work by monitoring transient pulses in conductance via
current–time traces as molecules translocate through the nanopore. The unique property of …