CRISPR/Cas9 therapeutics: progress and prospects
T Li, Y Yang, H Qi, W Cui, L Zhang, X Fu, X He… - … and Targeted Therapy, 2023 - nature.com
Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated
protein 9 (Cas9) gene-editing technology is the ideal tool of the future for treating diseases …
protein 9 (Cas9) gene-editing technology is the ideal tool of the future for treating diseases …
CRISPR-Cas guides the future of genetic engineering
The diversity, modularity, and efficacy of CRISPR-Cas systems are driving a
biotechnological revolution. RNA-guided Cas enzymes have been adopted as tools to …
biotechnological revolution. RNA-guided Cas enzymes have been adopted as tools to …
Unconstrained genome targeting with near-PAMless engineered CRISPR-Cas9 variants
Manipulation of DNA by CRISPR-Cas enzymes requires the recognition of a protospacer-
adjacent motif (PAM), limiting target site recognition to a subset of sequences. To remove …
adjacent motif (PAM), limiting target site recognition to a subset of sequences. To remove …
[HTML][HTML] Nanotechnology-based delivery of CRISPR/Cas9 for cancer treatment
Abstract CRISPR/Cas9 (Clustered Regularly Interspaced Short Palindromic Repeats-
associated protein 9) is a potent technology for gene-editing. Owing to its high specificity …
associated protein 9) is a potent technology for gene-editing. Owing to its high specificity …
Multiplexed CRISPR technologies for gene editing and transcriptional regulation
NS McCarty, AE Graham, L Studená… - Nature …, 2020 - nature.com
Multiplexed CRISPR technologies, in which numerous gRNAs or Cas enzymes are
expressed at once, have facilitated powerful biological engineering applications, vastly …
expressed at once, have facilitated powerful biological engineering applications, vastly …
The biology of CRISPR-Cas: backward and forward
F Hille, H Richter, SP Wong, M Bratovič, S Ressel… - Cell, 2018 - cell.com
In bacteria and archaea, clustered regularly interspaced short palindromic repeats
(CRISPR) and CRISPR-associated (Cas) proteins constitute an adaptive immune system …
(CRISPR) and CRISPR-associated (Cas) proteins constitute an adaptive immune system …
C2c2 is a single-component programmable RNA-guided RNA-targeting CRISPR effector
INTRODUCTION Almost all archaea and about half of bacteria possess clustered regularly
interspaced short palindromic repeat (CRISPR)–CRISPR-associated genes (Cas) adaptive …
interspaced short palindromic repeat (CRISPR)–CRISPR-associated genes (Cas) adaptive …
CRISPR–Cas9 structures and mechanisms
Many bacterial clustered regularly interspaced short palindromic repeats (CRISPR)–
CRISPR-associated (Cas) systems employ the dual RNA–guided DNA endonuclease Cas9 …
CRISPR-associated (Cas) systems employ the dual RNA–guided DNA endonuclease Cas9 …
RNA-targeting CRISPR–Cas systems
CRISPR–Cas is a widespread adaptive immune system in bacteria and archaea that
protects against viral infection by targeting specific invading nucleic acid sequences …
protects against viral infection by targeting specific invading nucleic acid sequences …
[HTML][HTML] Biology and applications of CRISPR systems: harnessing nature's toolbox for genome engineering
Bacteria and archaea possess a range of defense mechanisms to combat plasmids and viral
infections. Unique among these are the CRISPR-Cas (clustered regularly interspaced short …
infections. Unique among these are the CRISPR-Cas (clustered regularly interspaced short …