Coacervate droplets for synthetic cells

Z Lin, T Beneyton, JC Baret, N Martin - Small Methods, 2023 - Wiley Online Library
The design and construction of synthetic cells–human‐made microcompartments that mimic
features of living cells–have experienced a real boom in the past decade. While many efforts …

[HTML][HTML] Coacervates as models of membraneless organelles

NA Yewdall, AAM André, T Lu, E Spruijt - Current Opinion in Colloid & …, 2021 - Elsevier
Coacervates are condensed liquid-like droplets, usually formed with oppositely charged
polymeric molecules. They have been studied extensively in colloid and interface science …

Protocells: Milestones and recent advances

I Gözen, ES Köksal, I Põldsalu, L Xue, K Spustova… - Small, 2022 - Wiley Online Library
The origin of life is still one of humankind's great mysteries. At the transition between
nonliving and living matter, protocells, initially featureless aggregates of abiotic matter, gain …

Self-assembly of stabilized droplets from liquid–liquid phase separation for higher-order structures and functions

M Naz, L Zhang, C Chen, S Yang, H Dou… - Communications …, 2024 - nature.com
Dynamic microscale droplets produced by liquid–liquid phase separation (LLPS) have
emerged as appealing biomaterials due to their remarkable features. However, the …

Plant cell-inspired membranization of coacervate protocells with a structured polysaccharide layer

Y Ji, Y Lin, Y Qiao - Journal of the American Chemical Society, 2023 - ACS Publications
The design of compartmentalized colloids that exhibit biomimetic properties is providing
model systems for develo** synthetic cell-like entities (protocells). Inspired by the cell …

ATP‐responsive and ATP‐fueled self‐assembling systems and materials

J Deng, A Walther - Advanced Materials, 2020 - Wiley Online Library
Adenosine triphosphate (ATP) is a central metabolite that plays an indispensable role in
various cellular processes, from energy supply to cell‐to‐cell signaling. Nature has …

Synthetic cells revisited: Artificial cell construction using polymeric building blocks

V Maffeis, L Heuberger, A Nikoletić… - Advanced …, 2024 - Wiley Online Library
The exponential growth of research on artificial cells and organelles underscores their
potential as tools to advance the understanding of fundamental biological processes. The …

Signal processing and generation of bioactive nitric oxide in a model prototissue

S Liu, Y Zhang, X He, M Li, J Huang, X Yang… - Nature …, 2022 - nature.com
The design and construction of synthetic prototissues from integrated assemblies of artificial
protocells is an important challenge for synthetic biology and bioengineering. Here we …

Triggerable protocell capture in nanoparticle-caged coacervate microdroplets

N Gao, C Xu, Z Yin, M Li, S Mann - Journal of the American …, 2022 - ACS Publications
Controlling the dynamics of mixed communities of cell-like entities (protocells) provides a
step toward the development of higher-order cytomimetic behaviors in artificial cell …

Peptide-based coacervate protocells with cytoprotective metal–phenolic network membranes

L Jiang, Y Zeng, H Li, Z Lin, H Liu… - Journal of the …, 2023 - ACS Publications
Protocells have garnered considerable attention from cell biologists, materials scientists,
and synthetic biologists. Phase-separating coacervate microdroplets have emerged as a …