[HTML][HTML] Standards for methods utilizing environmental DNA for detection of fish species

L Shu, A Ludwig, Z Peng - Genes, 2020 - mdpi.com
Environmental DNA (eDNA) techniques are gaining attention as cost-effective, non-invasive
strategies for acquiring information on fish and other aquatic organisms from water samples …

A new era in palaeomicrobiology: prospects for ancient dental calculus as a long-term record of the human oral microbiome

C Warinner, C Speller… - … Transactions of the …, 2015 - royalsocietypublishing.org
The field of palaeomicrobiology is dramatically expanding thanks to recent advances in high-
throughput biomolecular sequencing, which allows unprecedented access to the …

Population genomics of the critically endangered kākāpō

N Dussex, T Van Der Valk, HE Morales, CW Wheat… - Cell Genomics, 2021 - cell.com
The kākāpō is a flightless parrot endemic to New Zealand. Once common in the archipelago,
only 201 individuals remain today, most of them descending from an isolated island …

Extraction of highly degraded DNA from ancient bones, teeth and sediments for high-throughput sequencing

N Rohland, I Glocke, A Aximu-Petri, M Meyer - Nature protocols, 2018 - nature.com
DNA preserved in ancient bones, teeth and sediments is typically highly fragmented and
present only in minute amounts. Here, we provide a highly versatile silica-based DNA …

Using eDNA to develop a national citizen science-based monitoring programme for the great crested newt (Triturus cristatus)

J Biggs, N Ewald, A Valentini, C Gaboriaud… - Biological …, 2015 - Elsevier
The use of environmental DNA (eDNA) is rapidly emerging as a potentially valuable survey
technique for rare or hard to survey freshwater organisms. For the great crested newt …

[HTML][HTML] Fish environmental DNA is more concentrated in aquatic sediments than surface water

CR Turner, KL Uy, RC Everhart - Biological Conservation, 2015 - Elsevier
Genetic identification of aqueous environmental DNA (eDNA) provides site occupancy
inferences for rare aquatic macrofauna that are often easier to obtain than direct …

Partial uracil–DNA–glycosylase treatment for screening of ancient DNA

N Rohland, E Harney, S Mallick… - … Transactions of the …, 2015 - royalsocietypublishing.org
The challenge of sequencing ancient DNA has led to the development of specialized
laboratory protocols that have focused on reducing contamination and maximizing the …

Manual and automated preparation of single-stranded DNA libraries for the sequencing of DNA from ancient biological remains and other sources of highly degraded …

MT Gansauge, A Aximu-Petri, S Nagel, M Meyer - Nature Protocols, 2020 - nature.com
It has been shown that highly fragmented DNA is most efficiently converted into DNA
libraries for sequencing if both strands of the DNA fragments are processed independently …

Genomic insights into the peopling of the Southwest Pacific

P Skoglund, C Posth, K Sirak, M Spriggs, F Valentin… - Nature, 2016 - nature.com
The appearance of people associated with the Lapita culture in the South Pacific around
3,000 years ago marked the beginning of the last major human dispersal to unpopulated …

Convergent regulatory evolution and loss of flight in paleognathous birds

TB Sackton, P Grayson, A Cloutier, Z Hu, JS Liu… - Science, 2019 - science.org
A core question in evolutionary biology is whether convergent phenotypic evolution is driven
by convergent molecular changes in proteins or regulatory regions. We combined …