The MTT assay application to measure the viability of spermatozoa: A variety of the assay protocols
K Buranaamnuay - Open veterinary journal, 2021 - ajol.info
Abstract The 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) reduction
assay is one of the methods used to evaluate the viability of sperm. In the assay, a …
assay is one of the methods used to evaluate the viability of sperm. In the assay, a …
Improving ICSI: A review from the spermatozoon perspective
M Simopoulou, L Gkoles, P Bakas… - Systems biology in …, 2016 - Taylor & Francis
Intracytoplasmic sperm injection (ICSI) is the most frequently applied method for fertilization
making the process of identifying the perfect spermatozoon fundamental. Herein we offer a …
making the process of identifying the perfect spermatozoon fundamental. Herein we offer a …
Microplastics hamper the fertilization success of a broadcast spawning bivalve through reducing gamete collision and gamete fusion efficiency
W Shi, S Sun, Y Han, Y Tang, W Zhou, W Zhang, X Du… - Aquatic Toxicology, 2022 - Elsevier
By employing external fertilization (broadcast spawning) as a mating strategy, the gametes
and subsequent fertilization of various marine invertebrates are directly subjected to …
and subsequent fertilization of various marine invertebrates are directly subjected to …
[HTML][HTML] Effect of zinc oxide nanoparticles on viability of human spermatozoa
A Barkhordari, S Hekmatimoghaddam… - Iranian journal of …, 2013 - ncbi.nlm.nih.gov
Background: The extensive use of different nanoparticles has raised great concerns about
their occupational and biological safety. Objective: The aim of this study was to evaluate the …
their occupational and biological safety. Objective: The aim of this study was to evaluate the …
Transgenerational effects of paternal exposures: the role of germline de novo mutations
Germline de novo mutations (DNMs) refer to spontaneous mutations arising during
gametogenesis, resulting in genetic changes within germ cells that are subsequently …
gametogenesis, resulting in genetic changes within germ cells that are subsequently …
based quantification of male fertility potential
BACKGROUND More than 70 million couples worldwide are affected by infertility, with male-
factor infertility accounting for about half of the cases. Semen analysis is critical for …
factor infertility accounting for about half of the cases. Semen analysis is critical for …
[HTML][HTML] Deletion of murine choline dehydrogenase results in diminished sperm motility
AR Johnson, CN Craciunescu, Z Guo, YW Teng… - The FASEB …, 2010 - ncbi.nlm.nih.gov
Choline dehydrogenase (CHDH) catalyzes the conversion of choline to betaine, an
important methyl donor and organic osmolyte. We have previously identified single …
important methyl donor and organic osmolyte. We have previously identified single …
Assessment of bovine sperm viability by MTT reduction assay
DM Aziz - Animal Reproduction Science, 2006 - Elsevier
The MTT reduction assay depends on the ability of metabolically active cells to reduce the
tetrazolium salt (3 [4, 5-dimethylthiazol-2-y1]-2, 5-diphenyltetrazolium bromide) to formazan …
tetrazolium salt (3 [4, 5-dimethylthiazol-2-y1]-2, 5-diphenyltetrazolium bromide) to formazan …
Effects of tempol and quercetin on human sperm function after cryopreservation
L Azadi, M Tavalaee, MR Deemeh, M Arbabian… - …, 2017 - ingentaconnect.com
BACKGROUND: Quercetin is a flavonoid with high reactive oxygen species (ROS)
scavenging and ion chelating activity. It also enhances the activity of antioxidant enzymes …
scavenging and ion chelating activity. It also enhances the activity of antioxidant enzymes …
Therapeutic effect of Fe2O3 nanoparticles combined with magnetic fluid hyperthermia on cultured liver cancer cells and xenograft liver cancers
S Yan, D Zhang, N Gu, J Zheng, A Ding… - … of Nanoscience and …, 2005 - ingentaconnect.com
The therapeutic effect of Fe2O3 nanoparticles combined with magnetic fluid hyperthermia
(MFH) on human hepatocarcinoma SMMC-7721 cells in vitro and xenograft liver cancer in …
(MFH) on human hepatocarcinoma SMMC-7721 cells in vitro and xenograft liver cancer in …