The nematode Caenorhabditis elegans has emerged as an important animal model in
various fields including neurobiology, developmental biology, and genetics. Characteristics …

Molecules that can specifically silence gene expression are powerful research tools. Much
effort has been put into the development of such molecules and has resulted in the creation …

Ageing is driven by a loss of cellular integrity. Given the major role of ubiquitin modifications
in cell function, here we assess the link between ubiquitination and ageing by quantifying …

Gene activity defines cell identity, drives intercellular communication, and underlies the
functioning of multicellular organisms. We present the single-cell resolution atlas of gene …

The recently completed Caenorhabditis elegans genome sequence allows application of
high-throughput (HT) approaches for phenotypic analyses using RNA interference (RNAi) …

Random transposon insertion libraries have proven invaluable in studying bacterial
genomes. Libraries that approach saturation must be large, with multiple insertions per …

Background RNA interference (RNAi) is a valuable reverse genetics tool to study gene
function in various organisms, including hemipteran insects such as aphids. Previous work …

We expressed SID-1, a transmembrane protein from Caenorhabditis elegans that is required
for systemic RNA interference (RNAi), in C. elegans neurons. This expression increased the …

In the era of functional genomics, the need for tools to perform large-scale targeted and
random mutagenesis is increasing. A potential tool is Agrobacterium-mediated fungal …

Background The linkage between duplicated chromosomes (sister chromatids) is
established during S phase by the action of cohesin, a multisubunit complex conserved from …