Functionalizing DNA origami to investigate and interact with biological systems

GA Knappe, EC Wamhoff, M Bathe - Nature Reviews Materials, 2023 - nature.com
DNA origami has emerged as a powerful method to generate DNA nanostructures with
dynamic properties and nanoscale control. These nanostructures enable complex …

Exploring giant unilamellar vesicle production for artificial cells—Current challenges and future directions

L Van de Cauter, L van Buren, GH Koenderink… - Small …, 2023 - Wiley Online Library
Creating an artificial cell from the bottom up is a long‐standing challenge and, while
significant progress has been made, the full realization of this goal remains elusive …

Bottom-up assembly of synthetic cells with a DNA cytoskeleton

K Jahnke, V Huth, U Mersdorf, N Liu, K Göpfrich - ACS nano, 2022 - ACS Publications
Cytoskeletal elements, like actin and myosin, have been reconstituted inside lipid vesicles
toward the vision to reconstruct cells from the bottom up. Here, we realize the de novo …

Modulating the lifetime of DNA motifs using visible light and small molecules

L Wimberger, FJ Rizzuto, JE Beves - Journal of the American …, 2023 - ACS Publications
Here we regulate the formation of dissipative assemblies built from DNA using a
merocyanine photoacid that responds to visible light. The operation of our system and the …

Building a community to engineer synthetic cells and organelles from the bottom-up

O Staufer, JA De Lora, E Bailoni, A Bazrafshan… - Elife, 2021 - elifesciences.org
Employing concepts from physics, chemistry and bioengineering,'learning-by-
building'approaches are becoming increasingly popular in the life sciences, especially with …

Modularized synthetic biology enabled intelligent biosensors

C Zhang, H Liu, X Li, F Xu, Z Li - Trends in Biotechnology, 2023 - cell.com
Biosensors that sense the concentration of a specified target and produce a specific signal
output have become important technology for biological analysis. Recently, intelligent …

Two‐Photon 3D Laser Printing Inside Synthetic Cells

T Abele, T Messer, K Jahnke, M Hippler… - Advanced …, 2022 - Wiley Online Library
Toward the ambitious goal of manufacturing synthetic cells from the bottom up, various
cellular components have already been reconstituted inside lipid vesicles. However, the …

Modulating lipid membrane morphology by dynamic DNA origami networks

J Yang, K Jahnke, L **n, X **g, P Zhan, A Peil… - Nano Letters, 2023 - ACS Publications
Membrane morphology and its dynamic adaptation regulate many cellular functions, which
are often mediated by membrane proteins. Advances in DNA nanotechnology have enabled …

Engineering DNA-based cytoskeletons for synthetic cells

K Jahnke, K Göpfrich - Interface Focus, 2023 - royalsocietypublishing.org
The development and bottom-up assembly of synthetic cells with a functional cytoskeleton
sets a major milestone to understand cell mechanics and to develop man-made machines …

Extracellular cues govern shape and cytoskeletal organization in giant unilamellar lipid vesicles

A Fink, CR Doll, A Yagüe Relimpio… - ACS Synthetic …, 2023 - ACS Publications
Spontaneous and induced front-rear polarization and a subsequent asymmetric actin
cytoskeleton is a crucial event leading to cell migration, a key process involved in a variety of …