Applications of CRISPR technologies in research and beyond

R Barrangou, JA Doudna - Nature biotechnology, 2016 - nature.com
Programmable DNA cleavage using CRISPR–Cas9 enables efficient, site-specific genome
engineering in single cells and whole organisms. In the research arena, versatile CRISPR …

Cryo-EM advances in RNA structure determination

H Ma, X Jia, K Zhang, Z Su - Signal Transduction and Targeted Therapy, 2022 - nature.com
Cryo-electron microscopy (cryo-EM) has emerged as an unprecedented tool to resolve
protein structures at atomic resolution. Structural insights of biological samples not …

Endosymbiotic theories for eukaryote origin

WF Martin, S Garg, V Zimorski - … Transactions of the …, 2015 - royalsocietypublishing.org
For over 100 years, endosymbiotic theories have figured in thoughts about the differences
between prokaryotic and eukaryotic cells. More than 20 different versions of endosymbiotic …

Structure of the R2 non-LTR retrotransposon initiating target-primed reverse transcription

ME Wilkinson, CJ Frangieh, RK Macrae, F Zhang - Science, 2023 - science.org
Non–long terminal repeat (non-LTR) retrotransposons, or long interspersed nuclear
elements (LINEs), are an abundant class of eukaryotic transposons that insert into genomes …

[HTML][HTML] Origins and evolution of viruses of eukaryotes: the ultimate modularity

EV Koonin, VV Dolja, M Krupovic - Virology, 2015 - Elsevier
Viruses and other selfish genetic elements are dominant entities in the biosphere, with
respect to both physical abundance and genetic diversity. Various selfish elements …

Engineering and optimization of systems, methods and compositions for sequence manipulation with functional domains

F Zhang, L Cong, RJ Platt, NE Sanjana - US Patent 8,999,641, 2015 - Google Patents
First worldwide family litigation filed litigation Critical https://patents. darts-ip. com/? family=
49883295&utm_source= google_patent&utm_medium= platform_link&utm_campaign …

[BUCH][B] Vital Question: Energy, Evolution, and the Origins of Complex Life

N Lane - 2015 - books.google.com
“One of the deepest, most illuminating books about the history of life to have been published
in recent years.”—The Economist The Earth teems with life: in its oceans, forests, skies and …

Classification and evolution of type II CRISPR-Cas systems

K Chylinski, KS Makarova, E Charpentier… - Nucleic acids …, 2014 - academic.oup.com
The CRISPR-Cas systems of archaeal and bacterial adaptive immunity are classified into
three types that differ by the repertoires of CRISPR-associated (cas) genes, the organization …

The spliceosome: the ultimate RNA chaperone and sculptor

P Papasaikas, J Valcárcel - Trends in biochemical sciences, 2016 - cell.com
The spliceosome, one of the most complex machineries of eukaryotic cells, removes intronic
sequences from primary transcripts to generate functional messenger and long noncoding …

Origin and evolution of spliceosomal introns

IB Rogozin, L Carmel, M Csuros, EV Koonin - Biology direct, 2012 - Springer
Evolution of exon-intron structure of eukaryotic genes has been a matter of long-standing,
intensive debate. The introns-early concept, later rebranded 'introns first'held that protein …