Sialic acid aptamer and RNA in situ hybridization-mediated proximity ligation assay for spatial imaging of glycoRNAs in single cells

W Guo, Y Ma, Q Mou, X Shao, M Lyu, V Garcia… - Nature …, 2025 - nature.com
Glycosylated RNAs (glycoRNAs) have recently emerged as a new class of molecules of
substantial interest owing to their potential roles in cellular processes and diseases …

Sugar Highs: Recent Notable Breakthroughs in Glycobiology

J Hu, DT Huynh, M Boyce - Biochemistry, 2024 - ACS Publications
Glycosylation is biochemically complex and functionally critical to a wide range of processes
and disease states, making it a vibrant area of contemporary research. Here, we highlight a …

N-glycosylated molecules act as a co-precipitant in RNA purification

S Kim, Y Choi, K Janssen, C Büll, BS Joshi, A Pomorski… - bioRxiv, 2024 - biorxiv.org
A recent ground-breaking study suggested that small RNA from mammalian cells can
undergo N-glycan modifications (termed glycoRNA). The discovery relied upon a metabolic …

Cell-Surface RNA Associates with Heparan Sulfate and RNA-Binding Proteins to Modulate Receptor-Ligand Interactions

Z Li, BS Joshi, RH Wijdeven, I Santos-Barriopedro… - bioRxiv, 2024 - biorxiv.org
Recent discoveries have shown the presence of RNA molecules on the cell surface, defying
the traditional view that RNA only functions intracellularly. However, it is not well understood …

Cell surface RNA expression modulates alveolar epithelial function

JK Abledu, CJ Herbst, R Brandt, A Kocak, P Ghosh… - bioRxiv, 2024 - biorxiv.org
Glycosylated RNA (glycoRNA) has recently emerged as a novel constituent of the glycocalyx
on cell surfaces, yet its biological functions remain largely unexplored. In this report, we …

A draft of human N-glycans of glycoRNA

M Bi, Z Zhang, T Wang, H Liang, Z Tian - bioRxiv, 2023 - biorxiv.org
In addition to the backbone molecules of proteins and lipids, RNAs have recently been
found to be N-glycosylated as well in cell models. Some overlap of N-glycans between RNA …