Synthetic genomes

W Zhang, LA Mitchell, JS Bader… - Annual review of …, 2020‏ - annualreviews.org
DNA synthesis technology has progressed to the point that it is now practical to synthesize
entire genomes. Quite a variety of methods have been developed, first to synthesize single …

A review on acoustic droplet ejection technology and system

Q Guo, X Su, X Zhang, M Shao, H Yu, D Li - Soft Matter, 2021‏ - pubs.rsc.org
The pace of change in chemical and biological research enabled by improved detection
systems demands fundamental liquid handling and sample preparation changes. The …

A versatile platform for locus-scale genome rewriting and verification

R Brosh, JM Laurent, R Ordoñez, E Huang… - Proceedings of the …, 2021‏ - pnas.org
Routine rewriting of loci associated with human traits and diseases would facilitate their
functional analysis. However, existing DNA integration approaches are limited in terms of …

Synthetic genomes with altered genetic codes

N Ostrov, A Nyerges, A Chiappino-Pepe… - Current Opinion in …, 2020‏ - Elsevier
The genetic code is the set of rules that define how information encoded in DNA is
interpreted into amino acids to make proteins. With few naturally occurring exceptions, the …

Superloser: A Plasmid Shuffling Vector for Saccharomyces cerevisiae with Exceedingly Low Background

MAB Haase, DM Truong… - G3: Genes, Genomes …, 2019‏ - academic.oup.com
Here we report a new plasmid shuffle vector for forcing budding yeast (Saccharomyces
cerevisiae) to incorporate a new genetic pathway in place of a native pathway–even an …

Fast acoustic droplet ejection based on annular array transducer

Y Huang, Y Zhang, W Wu, Y Wang, W Qiu, Z Zhang… - Ultrasonics, 2025‏ - Elsevier
Acoustic droplet ejection (ADE) has become the preferred method for liquid transfer in a
variety of applications including synthetic biology, genoty** and drug discovery …

From kilobases to megabases: design and delivery of large DNA constructs into mammalian genomes

FG Liberante, T Ellis - Current Opinion in Systems Biology, 2021‏ - Elsevier
As DNA synthesis has become cheaper, it has made assembly of larger and larger genes
possible. To fully realise this opportunity for a new era of synthetic biology in mammals, a …

DNA SYNTHESIS AND SYNTHETIC GENOME

ZX **e, RY Zhu, Y Liu, YJ Yuan - Nucleic Acids in Medicinal …, 2023‏ - Wiley Online Library
Synthetic genomics is a nascent field with technologies rapidly developed in the past
decade. In this chapter, we review the history of synthetic genomics and a variety of …

Methods and compositions for integration of a dna construct

DM Truong - US Patent App. 18/185,601, 2023‏ - Google Patents
US20230295668A1 - Methods and compositions for integration of a dna construct - Google
Patents US20230295668A1 - Methods and compositions for integration of a dna construct …

[PDF][PDF] Big DNA as a tool to dissect an age-related macular degeneration haplotype

JM Laurent, X Fu, S German, MT Maurano, K Zhang… - bioRxiv, 2018‏ - biorxiv.org
Abstract Age-related Macular Degeneration (AMD) is a leading cause of blindness in the
developed world, especially in aging populations, and is therefore an important target for …