Diffraction (X-ray, neutron and electron) and electron cryo-microscopy are powerful methods
to determine three-dimensional macromolecular structures, which are required to …

Membrane proteins are highly diverse in both structure and function and can, therefore,
present different challenges for structure determination. They are biologically important for …

The quality of diffraction data obtained using silver and molybdenum microsources has been
compared for six model compounds with a wide range of absorption factors. The …

Polyketide natural products constitute a broad class of compounds with diverse structural
features and biological activities. Their biosynthetic machinery, represented by type I …

We describe a general approach for refining protein structure models on the basis of cryo-
electron microscopy maps with near-atomic resolution. The method integrates Monte Carlo …

This Meeting Review describes the proceedings and conclusions from the inaugural
meeting of the Electron Microscopy Validation Task Force organized by the Unified Data …

The resolution of density maps from single particle analysis is usually measured in terms of
the highest spatial frequency to which consistent information has been obtained. This …

The peptide-loading complex (PLC) is a transient, multisubunit membrane complex in the
endoplasmic reticulum that is essential for establishing a hierarchical immune response …

Motivation Recent technological advances and computational developments have allowed
the reconstruction of Cryo-Electron Microscopy (cryo-EM) maps at near-atomic resolution …

The polyketide synthase (PKS) mega-enzyme assembly line uses a modular architecture to
synthesize diverse and bioactive natural products that often constitute the core structures or …