Bias in RNA‐seq library preparation: current challenges and solutions

H Shi, Y Zhou, E Jia, M Pan, Y Bai… - BioMed research …, 2021 - Wiley Online Library
Although RNA sequencing (RNA‐seq) has become the most advanced technology for
transcriptome analysis, it also confronts various challenges. As we all know, the workflow of …

Creating a functional single-chromosome yeast

Y Shao, N Lu, Z Wu, C Cai, S Wang, LL Zhang, F Zhou… - Nature, 2018 - nature.com
Eukaryotic genomes are generally organized in multiple chromosomes. Here we have
created a functional single-chromosome yeast from a Saccharomyces cerevisiae haploid …

Comparative population genomics in animals uncovers the determinants of genetic diversity

J Romiguier, P Gayral, M Ballenghien, A Bernard… - Nature, 2014 - nature.com
Genetic diversity is the amount of variation observed between DNA sequences from distinct
individuals of a given species. This pivotal concept of population genetics has implications …

Molecular tools and bumble bees: revealing hidden details of ecology and evolution in a model system

SH Woodard, JD Lozier, D Goulson… - Molecular …, 2015 - Wiley Online Library
Bumble bees are a longstanding model system for studies on behaviour, ecology and
evolution, due to their well‐studied social lifestyle, invaluable role as wild and managed …

Phylogenomic analyses support the position of turtles as the sister group of birds and crocodiles (Archosauria)

Y Chiari, V Cahais, N Galtier, F Delsuc - BMC biology, 2012 - Springer
Background The morphological peculiarities of turtles have, for a long time, impeded their
accurate placement in the phylogeny of amniotes. Molecular data used to address this major …

Adaptive protein evolution in animals and the effective population size hypothesis

N Galtier - PLoS genetics, 2016 - journals.plos.org
The rate at which genomes adapt to environmental changes and the prevalence of adaptive
processes in molecular evolution are two controversial issues in current evolutionary …

Evaluating methods for isolating total RNA and predicting the success of sequencing phylogenetically diverse plant transcriptomes

MTJ Johnson, EJ Carpenter, Z Tian, R Bruskiewich… - PloS one, 2012 - journals.plos.org
Next-generation sequencing plays a central role in the characterization and quantification of
transcriptomes. Although numerous metrics are purported to quantify the quality of RNA …

Preservation of RNA and DNA from mammal samples under field conditions

M Camacho‐Sanchez, P Burraco… - Molecular ecology …, 2013 - Wiley Online Library
Ecological and conservation genetics require sampling of organisms in the wild. Appropriate
preservation of the collected samples, usually by cryostorage, is key to the quality of the …

A phylogenomic supertree of birds

RT Kimball, CH Oliveros, N Wang, ND White, FK Barker… - Diversity, 2019 - mdpi.com
It has long been appreciated that analyses of genomic data (eg, whole genome sequencing
or sequence capture) have the potential to reveal the tree of life, but it remains challenging …

Crossing the Species Barrier: Genomic Hotspots of Introgression between Two Highly Divergent Ciona intestinalis Species

C Roux, G Tsagkogeorga, N Bierne… - Molecular biology and …, 2013 - academic.oup.com
Inferring a realistic demographic model from genetic data is an important challenge to gain
insights into the historical events during the speciation process and to detect molecular …