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Past, present, and future of CRISPR genome editing technologies
Genome editing has been a transformative force in the life sciences and human medicine,
offering unprecedented opportunities to dissect complex biological processes and treat the …
offering unprecedented opportunities to dissect complex biological processes and treat the …
Genome editing with CRISPR–Cas nucleases, base editors, transposases and prime editors
AV Anzalone, LW Koblan, DR Liu - Nature biotechnology, 2020 - nature.com
The development of new CRISPR–Cas genome editing tools continues to drive major
advances in the life sciences. Four classes of CRISPR–Cas-derived genome editing agents …
advances in the life sciences. Four classes of CRISPR–Cas-derived genome editing agents …
Programmable RNA detection with CRISPR-Cas12a
SR Rananaware, EK Vesco, GM Shoemaker… - Nature …, 2023 - nature.com
Cas12a, a CRISPR-associated protein complex, has an inherent ability to cleave DNA
substrates and is utilized in diagnostic tools to identify DNA molecules. We demonstrate that …
substrates and is utilized in diagnostic tools to identify DNA molecules. We demonstrate that …
Unconstrained genome targeting with near-PAMless engineered CRISPR-Cas9 variants
RT Walton, KA Christie, MN Whittaker, BP Kleinstiver - Science, 2020 - science.org
Manipulation of DNA by CRISPR-Cas enzymes requires the recognition of a protospacer-
adjacent motif (PAM), limiting target site recognition to a subset of sequences. To remove …
adjacent motif (PAM), limiting target site recognition to a subset of sequences. To remove …
The next generation of CRISPR–Cas technologies and applications
A Pickar-Oliver, CA Gersbach - Nature reviews Molecular cell biology, 2019 - nature.com
The prokaryote-derived CRISPR–Cas genome editing systems have transformed our ability
to manipulate, detect, image and annotate specific DNA and RNA sequences in living cells …
to manipulate, detect, image and annotate specific DNA and RNA sequences in living cells …
Exploring the trans‐cleavage activity of CRISPR‐Cas12a (cpf1) for the development of a universal electrochemical biosensor
An accurate, rapid, and cost‐effective biosensor for the quantification of disease biomarkers
is vital for the development of early‐diagnostic point‐of‐care systems. The recent discovery …
is vital for the development of early‐diagnostic point‐of‐care systems. The recent discovery …
CRISPR/Cas genome editing and precision plant breeding in agriculture
K Chen, Y Wang, R Zhang, H Zhang… - Annual review of plant …, 2019 - annualreviews.org
Enhanced agricultural production through innovative breeding technology is urgently
needed to increase access to nutritious foods worldwide. Recent advances in CRISPR/Cas …
needed to increase access to nutritious foods worldwide. Recent advances in CRISPR/Cas …
Engineered CRISPR-Cas9 nuclease with expanded targeting space
H Nishimasu, X Shi, S Ishiguro, L Gao, S Hirano… - Science, 2018 - science.org
The RNA-guided endonuclease Cas9 cleaves its target DNA and is a powerful genome-
editing tool. However, the widely used Streptococcus pyogenes Cas9 enzyme (SpCas9) …
editing tool. However, the widely used Streptococcus pyogenes Cas9 enzyme (SpCas9) …
CRISPR/Cas system: recent advances and future prospects for genome editing
H Manghwar, K Lindsey, X Zhang, S ** - Trends in plant science, 2019 - cell.com
Genome editing (GE) has revolutionized biological research through the new ability to
precisely edit the genomes of living organisms. In recent years, various GE tools have been …
precisely edit the genomes of living organisms. In recent years, various GE tools have been …
Evolved Cas9 variants with broad PAM compatibility and high DNA specificity
A key limitation of the use of the CRISPR–Cas9 system for genome editing and other
applications is the requirement that a protospacer adjacent motif (PAM) be present at the …
applications is the requirement that a protospacer adjacent motif (PAM) be present at the …