The MTT assay application to measure the viability of spermatozoa: A variety of the assay protocols

K Buranaamnuay - Open veterinary journal, 2021 - ajol.info
Abstract The 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) reduction
assay is one of the methods used to evaluate the viability of sperm. In the assay, a …

Impact of environmental contaminants on reproductive health of male domestic ruminants: a review

PR Guvvala, JP Ravindra, S Selvaraju - Environmental Science and …, 2020 - Springer
Environmental contaminants are gaining more attention in the livestock sector lately due to
their harmful effects on productivity and fertility of livestock. Recent research indicates that …

Antioxidant efficiency of lycopene on oxidative stress-induced damage in bovine spermatozoa

E Tvrdá, A Kováčik, E Tušimová, D Paál… - Journal of animal …, 2016 - Springer
Background Lycopene (LYC) is a natural carotenoid with powerful reactive oxygen species
(ROS) scavenging activities. The aim of this study was to investigate if lycopene has the …

The influence of macro-and microelements in seminal plasma on diluted boar sperm quality

MZ Pipan, J Mrkun, BJ Strajn, KP Vrtač, J Kos… - Acta Veterinaria …, 2017 - Springer
Background Growing evidence indicates that macro-and microelements in the seminal
plasma of humans and various domestic animals are of great importance due to their roles …

Could metal exposure affect sperm parameters of domestic ruminants? A meta-analysis

IM Ribeiro, AG de Azevedo Viana… - Animal Reproduction …, 2022 - Elsevier
During the last decade, environmental toxicants have been considered a potential cause for
declining sperm quality. Toxic metals are not easily degraded and may accumulate along …

[PDF][PDF] Time and dose-dependent effects of Viscum album quercus on rabbit spermatozoa motility and viability in vitro

M Halo Jr, P Massanyi, A Gren, A Lasak… - Physiological …, 2019 - biomed.cas.cz
The target of this study was to evaluate the effect of extract of the European mistletoe–
Viscum album quercus L. on spermatozoa motility and viability in vitro. The CASA system …

[PDF][PDF] In vitro assessment of gentamicin cytotoxicity on the selected mammalian cell line (Vero cells)

A Kovacik, E Tvrda, D Fulopova, P Cupka… - Adv. Res. Life …, 2017 - sciendo.com
The aim of this study was to evaluate the in vitro cytotoxicity of different concentrations (500-
7500 μg/mL) of gentamicin-GENT (aminoglycoside antibiotic) on the selected mammalian …

[HTML][HTML] The effect of non-thermal plasma on the structural and functional characteristics of human spermatozoa

E Tvrdá, D Lovíšek, S Kyzek, D Kováčik… - International Journal of …, 2021 - mdpi.com
Significant antibacterial properties of non-thermal plasma (NTP) have converted this
technology into a promising alternative to the widespread use of antibiotics in assisted …

[PDF][PDF] Short term iron overload injection alters reproduction organ and sperm quality in male mice

M Syamsunarno, R Widyastuti… - Adv. Anim …, 2021 - nexusacademicpublishers.com
Background: Iron is an essential substrate in the body and needed in a small amount. In iron
overload condition, such as patients who undergo routine blood transfusion for a long …

Impact of elemental iron on human spermatozoa and mouse embryonic development in a defined synthetic culture medium

SM Naes, O Basri, F Ismail, GA Ata'Allah, SK Idris… - Reproductive …, 2017 - Elsevier
There is a paucity of studies on effect of iron in embryo culture procedures. This study aims
to ascertain the optimal, tolerance and toxic levels of iron in a protein-free embryo culture …