Pseudomonas as Versatile Aromatics Cell Factory

T Schwanemann, M Otto, N Wierckx… - Biotechnology …, 2020 - Wiley Online Library
Aromatics and their derivatives are valuable chemicals with a plethora of important
applications and thus play an integral role in modern society. Their current production relies …

Methodologies for preparation of prokaryotic extracts for cell-free expression systems

SD Cole, AE Miklos, AC Chiao, ZZ Sun… - Synthetic and Systems …, 2020 - Elsevier
Cell-free systems that mimic essential cell functions, such as gene expression, have
dramatically expanded in recent years, both in terms of applications and widespread …

Design and optimization of a cell-free atrazine biosensor

AD Silverman, U Akova, KK Alam, MC Jewett… - ACS synthetic …, 2020 - ACS Publications
Recent advances in cell-free synthetic biology have spurred the development of in vitro
molecular diagnostics that serve as effective alternatives to whole-cell biosensors. However …

Improving cell-free glycoprotein synthesis by characterizing and enriching native membrane vesicles

JM Hershewe, KF Warfel, SM Iyer, JA Peruzzi… - Nature …, 2021 - nature.com
Cell-free gene expression (CFE) systems from crude cellular extracts have attracted much
attention for biomanufacturing and synthetic biology. However, activating membrane …

Screening putative polyester polyurethane degrading enzymes with semi-automated cell-free expression and nitrophenyl probes

A Ahsan, D Wagner, VA Varaljay, V Roman… - Synthetic …, 2024 - academic.oup.com
Cell-free expression (CFE) has shown recent utility in prototy** enzymes for discovery
efforts. In this work, CFE is demonstrated as an effective tool to screen putative polyester …

Optimization of heavy metal sensors based on transcription factors and cell-free expression systems

K Beabout, CB Bernhards, M Thakur… - ACS Synthetic …, 2021 - ACS Publications
Many bacterial mechanisms for highly specific and sensitive detection of heavy metals and
other hazards have been reengineered to serve as sensors. In some cases, these sensors …

Marinobacter: A case study in bioelectrochemical chassis evaluation

LJ Bird, RL Mickol, BJ Eddie, M Thakur… - Microbial …, 2023 - Wiley Online Library
The junction of bioelectrochemical systems and synthetic biology opens the door to many
potentially groundbreaking technologies. When develo** these possibilities, choosing the …

Engineering a Synthetic Dopamine-Responsive Riboswitch for In Vitro Biosensing

SV Harbaugh, AD Silverman, YG Chushak… - ACS synthetic …, 2022 - ACS Publications
The detection of chemicals using natural allosteric transcription factors is a powerful strategy
for point-of-use molecular sensing, particularly using fieldable cell-free gene expression …

Detection of bile acids in complex matrices using a transcription factor-based biosensor

K Beabout, AM Ehrenworth Breedon… - ACS Biomaterials …, 2022 - ACS Publications
Bile acids play an important role in digestion and human health, are found throughout the
gastrointestinal tract, and are excreted in feces. Therefore, bile acids are promising …

Assessment of colorimetric reporter enzymes in the pure system

CE Sharpes, JB McManus, SM Blum… - ACS Synthetic …, 2021 - ACS Publications
Colorimetric reporter enzymes are useful for generating eye-readable biosensor readouts
that do not require a device to interpret, an attractive property for applications in remote or …