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Pseudomonas as Versatile Aromatics Cell Factory
Aromatics and their derivatives are valuable chemicals with a plethora of important
applications and thus play an integral role in modern society. Their current production relies …
applications and thus play an integral role in modern society. Their current production relies …
Methodologies for preparation of prokaryotic extracts for cell-free expression systems
Cell-free systems that mimic essential cell functions, such as gene expression, have
dramatically expanded in recent years, both in terms of applications and widespread …
dramatically expanded in recent years, both in terms of applications and widespread …
Design and optimization of a cell-free atrazine biosensor
Recent advances in cell-free synthetic biology have spurred the development of in vitro
molecular diagnostics that serve as effective alternatives to whole-cell biosensors. However …
molecular diagnostics that serve as effective alternatives to whole-cell biosensors. However …
Improving cell-free glycoprotein synthesis by characterizing and enriching native membrane vesicles
Cell-free gene expression (CFE) systems from crude cellular extracts have attracted much
attention for biomanufacturing and synthetic biology. However, activating membrane …
attention for biomanufacturing and synthetic biology. However, activating membrane …
Screening putative polyester polyurethane degrading enzymes with semi-automated cell-free expression and nitrophenyl probes
Cell-free expression (CFE) has shown recent utility in prototy** enzymes for discovery
efforts. In this work, CFE is demonstrated as an effective tool to screen putative polyester …
efforts. In this work, CFE is demonstrated as an effective tool to screen putative polyester …
Optimization of heavy metal sensors based on transcription factors and cell-free expression systems
Many bacterial mechanisms for highly specific and sensitive detection of heavy metals and
other hazards have been reengineered to serve as sensors. In some cases, these sensors …
other hazards have been reengineered to serve as sensors. In some cases, these sensors …
Marinobacter: A case study in bioelectrochemical chassis evaluation
The junction of bioelectrochemical systems and synthetic biology opens the door to many
potentially groundbreaking technologies. When develo** these possibilities, choosing the …
potentially groundbreaking technologies. When develo** these possibilities, choosing the …
Engineering a Synthetic Dopamine-Responsive Riboswitch for In Vitro Biosensing
SV Harbaugh, AD Silverman, YG Chushak… - ACS synthetic …, 2022 - ACS Publications
The detection of chemicals using natural allosteric transcription factors is a powerful strategy
for point-of-use molecular sensing, particularly using fieldable cell-free gene expression …
for point-of-use molecular sensing, particularly using fieldable cell-free gene expression …
Detection of bile acids in complex matrices using a transcription factor-based biosensor
Bile acids play an important role in digestion and human health, are found throughout the
gastrointestinal tract, and are excreted in feces. Therefore, bile acids are promising …
gastrointestinal tract, and are excreted in feces. Therefore, bile acids are promising …
Assessment of colorimetric reporter enzymes in the pure system
CE Sharpes, JB McManus, SM Blum… - ACS Synthetic …, 2021 - ACS Publications
Colorimetric reporter enzymes are useful for generating eye-readable biosensor readouts
that do not require a device to interpret, an attractive property for applications in remote or …
that do not require a device to interpret, an attractive property for applications in remote or …