[HTML][HTML] Engineering enzyme access tunnels
Enzymes are efficient and specific catalysts for many essential reactions in biotechnological
and pharmaceutical industries. Many times, the natural enzymes do not display the catalytic …
and pharmaceutical industries. Many times, the natural enzymes do not display the catalytic …
Computational design of enzymes for biotechnological applications
Enzymes are the natural catalysts that execute biochemical reactions upholding life. Their
natural effectiveness has been fine-tuned as a result of millions of years of natural evolution …
natural effectiveness has been fine-tuned as a result of millions of years of natural evolution …
CAVER Analyst 2.0: analysis and visualization of channels and tunnels in protein structures and molecular dynamics trajectories
Motivation Studying the transport paths of ligands, solvents, or ions in transmembrane
proteins and proteins with buried binding sites is fundamental to the understanding of their …
proteins and proteins with buried binding sites is fundamental to the understanding of their …
Caver Web 1.0: identification of tunnels and channels in proteins and analysis of ligand transport
Caver Web 1.0 is a web server for comprehensive analysis of protein tunnels and channels,
and study of the ligands' transport through these transport pathways. Caver Web is the first …
and study of the ligands' transport through these transport pathways. Caver Web is the first …
Structure-oriented engineering of amidase: modification of twisted access tunnel for efficient synthesis of 2-chloronicotinic acid
ZM Wu, F **e, W Zheng, CF Liu, CP Lin, RC Zheng… - ACS …, 2023 - ACS Publications
Amidases are robust biocatalysts for the industrial synthesis of carboxylic acids. Amidase-
catalyzed hydrolysis of 2-chloronicotinamide is regarded as a promising route for 2 …
catalyzed hydrolysis of 2-chloronicotinamide is regarded as a promising route for 2 …
Flexibility regulation of loops surrounding the tunnel entrance in cytochrome P450 enhanced substrate access substantially
In recent years, the engineering of flexible loops to improve enzyme properties has gained
attention in biocatalysis. Herein, we report a loop engineering strategy to improve the …
attention in biocatalysis. Herein, we report a loop engineering strategy to improve the …
[HTML][HTML] Tunnel engineering of gas-converting enzymes for inhibitor retardation and substrate acceleration
SM Kim, SH Kang, BW Jeon, YH Kim - Bioresource Technology, 2024 - Elsevier
Carbon monoxide dehydrogenase (CODH), formate dehydrogenase (FDH), hydrogenase
(H2ase), and nitrogenase (N2ase) are crucial enzymatic catalysts that facilitate the …
(H2ase), and nitrogenase (N2ase) are crucial enzymatic catalysts that facilitate the …
Substrate inhibition by the blockage of product release and its control by tunnel engineering
Substrate inhibition is the most common deviation from Michaelis–Menten kinetics, occurring
in approximately 25% of known enzymes. It is generally attributed to the formation of an …
in approximately 25% of known enzymes. It is generally attributed to the formation of an …
The modular structure of α/β‐hydrolases
The α/β‐hydrolase fold family is highly diverse in sequence, structure and biochemical
function. To investigate the sequence–structure–function relationships, the Lipase …
function. To investigate the sequence–structure–function relationships, the Lipase …
Rational enzyme design by reducing the number of hotspots and library size
Z Qin, B Yuan, G Qu, Z Sun - Chemical Communications, 2024 - pubs.rsc.org
Biocatalysts that are eco-friendly, sustainable, and highly specific have great potential for
applications in the production of fine chemicals, food, detergents, biofuels, pharmaceuticals …
applications in the production of fine chemicals, food, detergents, biofuels, pharmaceuticals …