CRISPR screens are a powerful source of biological discovery, enabling the unbiased
interrogation of gene function in a wide range of applications and species. In pooled …

Multiplexed CRISPR technologies, in which numerous gRNAs or Cas enzymes are
expressed at once, have facilitated powerful biological engineering applications, vastly …

The majority of therapies that target individual proteins rely on specific activity-modulating
interactions with the target protein—for example, enzyme inhibition or ligand blocking …

A general approach for heritably altering gene expression has the potential to enable many
discovery and therapeutic efforts. Here, we present CRISPRoff—a programmable epigenetic …

CRISPOR. org is a web tool for genome editing experiments with the CRISPR–Cas9 system.
It finds guide RNAs in an input sequence and ranks them according to different scores that …

Meningiomas are the most common primary intracranial tumors. There are no effective
medical therapies for meningioma patients, and new treatments have been encumbered by …

The creation of genome-wide libraries for CRISPR knockout (CRISPRko), interference
(CRISPRi), and activation (CRISPRa) has enabled the systematic interrogation of gene …

Altered microglial states affect neuroinflammation, neurodegeneration, and disease but
remain poorly understood. Here, we report 194,000 single-nucleus microglial transcriptomes …

Regulation of cytokine production in stimulated T cells can be disrupted in autoimmunity,
immunodeficiencies, and cancer. Systematic discovery of stimulation-dependent cytokine …

Ribosome profiling has revealed pervasive but largely uncharacterized translation outside of
canonical coding sequences (CDSs). In this work, we exploit a systematic CRISPR-based …