The concept of an epigenetic landscape describing potential cellular fates arising from
pluripotent cells, first advanced by Conrad Waddington, has evolved in light of experiments …

This year is the tenth anniversary of the publication in this journal of a model suggesting the
existence of'tumour progenitor genes'. These genes are epigenetically disrupted at the …

Identifying the relationships between chromosome structures, nuclear bodies, chromatin
states and gene expression is an overarching goal of nuclear-organization studies …

We perform a thorough analysis of RNA velocity methods, with a view towards
understanding the suitability of the various assumptions underlying popular …

It has long been the dream of biologists to map gene expression at the single-cell level. With
such data one might track heterogeneous cell sub-populations, and infer regulatory …

Cell-to-cell variation is a universal feature of life that affects a wide range of biological
phenomena, from developmental plasticity, to tumour heterogeneity. Although recent …

Cytosine methylation is a DNA modification generally associated with transcriptional
silencing. Factors that regulate methylation have been linked to human disease, yet how …

We report scM&T-seq, a method for parallel single-cell genome-wide methylome and
transcriptome sequencing that allows for the discovery of associations between …

Visualization of the transcriptome and the nuclear organization in situ has been challenging
for single-cell analysis. Here, we demonstrate a multiplexed single-molecule in situ method …

The regulative capability of single cells to give rise to all primary embryonic lineages is
termed pluripotency. Observations of fluctuating gene expression and phenotypic …