This review encompasses the most important advances in liver functions and hepatotoxicity
and analyzes which mechanisms can be studied in vitro. In a complex architecture of nested …

Introduction: The sandwich-cultured hepatocyte (SCH) model has become an invaluable in
vitro tool for studying hepatic drug transport, metabolism, biliary excretion and toxicity. The …

In an effort to reconstruct the cellular polarity normally found in the liver, adult rat
hepatocytes were sandwiched between two layers of hydrated rat tail tendon collagen …

The influence of extracellular matrix configuration on the tissue-specific function of cultured
hepatocytes was investigated. Adult rat hepatocytes sandwiched between two layers of …

Over the past few decades, the use of cell microencapsulation technology has been
promoted for a wide range of applications as sustained drug delivery systems or as cells …

Standard human embryonic stem (HES) cell cryopreservation methodologies, including slow
freezing and vitrification of colonies in suspension, are plagued by poor viability and high …

Drug discovery and development consists of a series of processes starting with the
demonstration of pharmacologica l effects in experimental cell and animal models and …

Cryopreservation in liquid nitrogen is presently the only way for long-term storage of isolated
hepatocytes. Freeze–thaw conditions are not well defined yet; the most critical parameters …

5,030,225 7/1991 Aebischer et a1, 606/152 A bioarti? cial extracellular matrix for use in
tissue regen 5,068,224 11/1991 Fryklund et al.. 514/21 eration or replacement is provided …

The use of hepatocyte cultures is well established for the study of drug–drug interactions.
However, the major hindrance for the use of human hepatocyte cultures is that human …