In-cell structural biology aims at extracting structural information about proteins or nucleic
acids in their native, cellular environment. This emerging field holds great promise and is …

Abstract Small GTPases including Ras, Rho, Rab, Arf, and Ran are omnipresent molecular
switches in regulating key cellular functions. Their dysregulation is a therapeutic target for …

Cellular processes are the product of interactions between biomolecules, which associate to
form biologically active complexes. These interactions are mediated by intermolecular …

Due to the lack of genetically encoded probes for fluorine-19 nuclear magnetic resonance
spectroscopy (19F NMR), its utility for probing eukaryotic membrane protein dynamics is …

NMR spectroscopy has been applied to cells and tissues analysis since its beginnings, as
early as 1950. We have attempted to gather here in a didactic fashion the broad diversity of …

Traditionally, structural biologists approach the complexity of cellular proteomes in a
reductionist manner. Proteomes are fractionated, their molecular components purified and …

Biomolecular nuclear magnetic resonance (NMR) spectroscopy and artificial intelligence
(AI) have a burgeoning synergy. Deep learning-based structural predictors have forever …

Proteins have dynamic structures that undergo chain motions on time scales spanning from
picoseconds to seconds. Resolving the resultant conformational heterogeneity is essential …

Homologous enzymes often exhibit different catalytic rates despite a fully conserved active
site. The canonical view is that an enzyme sequence defines its structure and function and …

Large biomolecular systems are at the heart of many essential cellular processes. The
dynamics and energetics of an increasing number of these systems are being studied by …