Life in Multi-Extreme Environments: Brines, Osmotic and Hydrostatic Pressure─ A Physicochemical View

JM Knop, S Mukherjee, MW Jaworek… - Chemical …, 2022 - ACS Publications
Elucidating the details of the formation, stability, interactions, and reactivity of biomolecular
systems under extreme environmental conditions, including high salt concentrations in …

Effects of Crowding and Cosolutes on Biomolecular Function at Extreme Environmental Conditions

J Peters, R Oliva, A Caliò, P Oger, R Winter - Chemical Reviews, 2023 - ACS Publications
The extent of the effect of cellular crowding and cosolutes on the functioning of proteins and
cells is manifold and includes the stabilization of the biomolecular systems, the excluded …

Improving laccase thermostability with aqueous natural deep eutectic solvents

AE Delorme, JM Andanson, V Verney - International Journal of Biological …, 2020 - Elsevier
The wide-spread use of laccases in industry is often limited due to the enzyme inactivation
over time at conditions which exceeds the operating conditions of the enzymes, which are …

Highly productive oxidative biocatalysis in continuous flow by enhancing the aqueous equilibrium solubility of oxygen

MR Chapman, SC Cosgrove, NJ Turner… - Angewandte Chemie …, 2018 - Wiley Online Library
We report a simple, mild, and synthetically clean approach to accelerate the rate of
enzymatic oxidation reactions by a factor of up to 100 when compared to conventional batch …

Boosting the kinetic efficiency of formate dehydrogenase by combining the effects of temperature, high pressure and co-solvent mixtures

MW Jaworek, NF Gajardo-Parra, G Sadowski… - Colloids and Surfaces B …, 2021 - Elsevier
The application of co-solvents and high pressure has been shown to be an efficient means
to modify the kinetics of enzyme-catalyzed reactions without compromising enzyme stability …

Osmolytes resist against harsh osmolarity: Something old something new

SMH Khatibi, FZ Vahed, S Sharifi, M Ardalan… - Biochimie, 2019 - Elsevier
From the halophilic bacteria to human, cells have to survive under the stresses of harsh
environments. Hyperosmotic stress is a process that triggers cell shrinkage, oxidative stress …

Enzyme‐catalyzed synthesis of esters in water

L Mestrom, JGR Claessen, U Hanefeld - ChemCatChem, 2019 - Wiley Online Library
MsAcT catalyzes the esterification of primary alcohols in water. When utilizing acid and
alcohol as starting materials low yields dictated by thermodynamics were observed …

Solvents and detergents compatible with enzyme kinetic studies of cholinesterases

D Sands, A Davis, S Banfield, IR Pottie… - Chemico-Biological …, 2023 - Elsevier
Abstract Acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) are enzymes that
serve a wide range of physiological functions including the hydrolysis of the neurotransmitter …

Evodiamine in several binary aqueous co-solvents: Solubility measurement and modeling, Hansen solubility parameter, preferential solvation and apparent …

C Zhang, A Farajtabar, A Jouyban, WE Acree Jr… - Journal of Molecular …, 2021 - Elsevier
Equilibrium solubilities of evodiamine were determined in four aqueous mixtures of
methanol, isopropanol, acetone and ethanol over their full mass fraction range via the shake …

Harnessing pressure-axis experiments to explore volume fluctuations, conformational substates, and solvation of biomolecular systems

R Oliva, R Winter - The Journal of Physical Chemistry Letters, 2022 - ACS Publications
Intrinsic thermodynamic fluctuations within biomolecules are crucial for their function, and
flexibility is one of the strategies that evolution has developed to adapt to extreme …