A guide to single-particle tracking

F Simon, LE Weiss, S van Teeffelen - Nature Reviews Methods Primers, 2024 - nature.com
Individual proteins and protein complexes undergo various motion types, including free
diffusion, confinement, subdiffusion and directed motion. Different motion behaviours reflect …

MINFLUX reveals dynein step** in live neurons

JM Schleske, J Hubrich, JO Wirth, E D'Este… - Proceedings of the …, 2024 - pnas.org
Dynein is the primary molecular motor responsible for retrograde intracellular transport of a
variety of cargoes, performing successive nanometer-sized steps within milliseconds. Due to …

Cryo-EM Reveals the Mechanochemical Cycle of Reactive Full-length Human Dynein-1

P Chai, J Yang, IC Geohring, SM Markus, Y Wang… - bioRxiv, 2024 - biorxiv.org
Dynein-mediated cargo transport plays pivotal roles in diverse cellular activities. Central to
all these cellular processes is dynein's ATP-dependent mechanochemical cycle. Here, we …

Multiple kinesins speed up cargo transport in crowded environments by sharing load

YT Huang, M Tomishige, SP Gross, PY Lai… - Communications …, 2025 - nature.com
Kinesin motors transport cargoes along microtubules inside of cells. Although it is well
known that the cargoes are typically carried by multiple kinesins and that the more motors …

MINFLUX Tracking of Endogenous Dynein in Live Neurons

JM Schleske - 2024 - archiv.ub.uni-heidelberg.de
MINFLUX represents a novel class of optical nanoscopy techniques, enabling tracking
measurements with nanometer/millisecond spatiotemporal precision by requiring …

A microscope for observing intracellular transport with molecular resolution on molecular timescales

JJ Axelrod, GE Marti, S Chu - Laser Science, 2024 - opg.optica.org
A microscope for observing intracellular transport with molecular resolution on molecular
timescales Page 1 A microscope for observing intracellular transport with molecular resolution …