Single-molecule spectroscopy is a powerful method for studying the physics of molecular
systems, particularly biomolecules, such as proteins and nucleic acids. By avoiding …

The regulation of cell motility is central to living systems. Consequently, cell migration
assays are some of the most frequently used in vitro assays. This article provides a …

To date, studies on the thermodynamic and kinetic processes that underlie biological
function and nanomachine actuation in biological-and biology-inspired molecular constructs …

Spatiotemporal control over crystal nucleation and growth is of fundamental interest for
understanding how organisms assemble high-performance biominerals, and holds …

In this work, the kinetics of short, fully complementary oligonucleotides are investigated at
the single-molecule level. Constructs 6–9 bp in length exhibit single exponential kinetics …

Single-molecule Förster resonance energy transfer (smFRET) has become a versatile and
widespread method to probe nanoscale conformation and dynamics. However, current …

Proteinaceous nanometer-scale pores are ubiquitous in biology. The canonical ionic
channels (eg, those that transport Na+, K+, Ca 2+, and Cl− across cell membranes) play key …

To our knowledge, we have developed a novel temperature-jump optical tweezers setup
that changes the temperature locally and rapidly. It uses a heating laser with a wavelength …

Pulsed temperature-jump (T-jump) spectroscopy with infrared (IR) detection has been widely
used to study biophysical processes occurring from nanoseconds to∼ 1 ms with structural …

The evolution of single-molecule and-particle fluorescence microscopy imaging techniques
for the investigation of chemical reactions has yielded achievements at all fronts, from …