The regulated transcription of genes determines cell identity and function. Recent structural
studies have elucidated mechanisms that govern the regulation of transcription by RNA …

The eukaryotic transcription apparatus synthesizes a staggering diversity of RNA molecules.
The labour of nuclear gene transcription is, therefore, divided among multiple DNA …

INTRODUCTION RNA polymerase II (Pol II)–mediated transcription initiation requires
assembly of a preinitiation complex (PIC), during which the 14-subunit transcription factor IID …

The landmark 1969 discovery of nuclear RNA polymerases I, II and III in diverse eukaryotes
represented a major turning point in the field that, with subsequent elucidation of the distinct …

Transcription of eukaryotic protein-coding genes commences with the assembly of a
conserved initiation complex, which consists of RNA polymerase II (Pol II) and the general …

Regulatory factor binding to genomic DNA protects the underlying sequence from cleavage
by DNase I, leaving nucleotide-resolution footprints. Using genomic DNase I footprinting …

Nuclear small RNA pathways safeguard genome integrity by establishing transcription-
repressing heterochromatin at transposable elements. This inevitably also targets the …

In eukaryotic transcription initiation, a large multi-subunit pre-initiation complex (PIC) that
assembles at the core promoter is required for the opening of the duplex DNA and …

Transcription and regulation of genes originate from transcription pre-initiation complexes
(PICs). Their structural and positional organization across eukaryotic genomes is unknown …

The conserved co-activator complex Mediator enables regulated transcription initiation by
RNA polymerase (Pol) II. Here we reconstitute an active 15-subunit core Mediator (cMed) …