Myosin motors power movements on actin filaments, whereas dynein and kinesin motors
power movements on microtubules. The mechanisms of these motor proteins differ, but, in …

Fuelled by ATP hydrolysis, dyneins generate force and movement on microtubules in a
wealth of biological processes, including ciliary beating, cell division and intracellular …

The axoneme is the main extracellular part of cilia and flagella in eukaryotes. It consists of a
microtubule cytoskeleton, which normally comprises nine doublets. In motile cilia, dynein …

The α-helical coiled coil is one of the principal subunit oligomerization motifs in proteins. Its
most characteristic feature is a heptad repeat pattern of primarily apolar residues that …

Several enzyme complexes drive cellular movements by coupling free energy-liberating
chemical reactions to the production of mechanical work1–3. A key goal in the study of these …

Dynein ATPases are microtubule motors that are critical to diverse processes such as
vesicle transport and the beating of sperm tails; however, their mechanism of force …

Kinesin is a microtubule-activated ATPase thought to transport membrane-bounded
organelles along MTs. To illuminate the structural basis for this function, EM was used to …

Publisher Summary This chapter provides a rationale for the design and interpretation of
transient kinetic experiments to establish enzyme pathways by direct analysis of individual …

The ATPase rate of kinesin isolated from bovine brain by the method of SA Kuznetsov and VI
Gelfand [(1986) Proc. Natl. Acad. Sci. USA 83, 8530-8534)] is stimulated 1000-fold by …

The role of cytoplasmic dynein in microtubule-based organelle transport was examined
using a reconstituted assay developed from chick embryo fibroblasts. Factors present in a …