ATM, ATR, and DNA-PK: The Trinity at the Heart of the DNA Damage Response
In vertebrate cells, the DNA damage response is controlled by three related kinases: ATM,
ATR, and DNA-PK. It has been 20 years since the cloning of ATR, the last of the three to be …
ATR, and DNA-PK. It has been 20 years since the cloning of ATR, the last of the three to be …
A decade of advances in transposon-insertion sequencing
It has been 10 years since the introduction of modern transposon-insertion sequencing (TIS)
methods, which combine genome-wide transposon mutagenesis with high-throughput …
methods, which combine genome-wide transposon mutagenesis with high-throughput …
Identification and characterization of essential genes in the human genome
Large-scale genetic analysis of lethal phenotypes has elucidated the molecular
underpinnings of many biological processes. Using the bacterial clustered regularly …
underpinnings of many biological processes. Using the bacterial clustered regularly …
Genome-wide recessive genetic screening in mammalian cells with a lentiviral CRISPR-guide RNA library
H Koike-Yusa, Y Li, EP Tan… - Nature …, 2014 - nature.com
Identification of genes influencing a phenotype of interest is frequently achieved through
genetic screening by RNA interference (RNAi) or knockouts. However, RNAi may only …
genetic screening by RNA interference (RNAi) or knockouts. However, RNAi may only …
Gene essentiality and synthetic lethality in haploid human cells
Although the genes essential for life have been identified in less complex model organisms,
their elucidation in human cells has been hindered by technical barriers. We used extensive …
their elucidation in human cells has been hindered by technical barriers. We used extensive …
Ebola virus entry requires the cholesterol transporter Niemann–Pick C1
Infections by the Ebola and Marburg filoviruses cause a rapidly fatal haemorrhagic fever in
humans for which no approved antivirals are available. Filovirus entry is mediated by the …
humans for which no approved antivirals are available. Filovirus entry is mediated by the …
The BioGRID interaction database: 2015 update
A Chatr-Aryamontri, BJ Breitkreutz… - Nucleic acids …, 2015 - academic.oup.com
Abstract The Biological General Repository for Interaction Datasets (BioGRID:
http://thebiogrid. org) is an open access database that houses genetic and protein …
http://thebiogrid. org) is an open access database that houses genetic and protein …
Human haploid cell genetics reveals roles for lipid metabolism genes in nonapoptotic cell death
Little is known about the regulation of nonapoptotic cell death. Using massive insertional
mutagenesis of haploid KBM7 cells we identified nine genes involved in small-molecule …
mutagenesis of haploid KBM7 cells we identified nine genes involved in small-molecule …
Gene regulation by the act of long non-coding RNA transcription
Long non-protein-coding RNAs (lncRNAs) are proposed to be the largest transcript class in
the mouse and human transcriptomes. Two important questions are whether all lncRNAs are …
the mouse and human transcriptomes. Two important questions are whether all lncRNAs are …
Exposure of phosphatidylserine on the cell surface
Phosphatidylserine (PtdSer) is a phospholipid that is abundant in eukaryotic plasma
membranes. An ATP-dependent enzyme called flippase normally keeps PtdSer inside the …
membranes. An ATP-dependent enzyme called flippase normally keeps PtdSer inside the …