[HTML][HTML] Extracellular matrix requirements for gastrointestinal organoid cultures

S Rezakhani, N Gjorevski, MP Lutolf - Biomaterials, 2021 - Elsevier
Organoids are a new class of biological model systems that have garnered significant
interest in the life sciences. When provided with the proper 3D matrix and biochemical …

Primary cell-derived intestinal models: recapitulating physiology

JS Dutton, SS Hinman, R Kim, Y Wang… - Trends in …, 2019 - cell.com
The development of physiologically relevant intestinal models fueled by breakthroughs in
primary cell-culture methods has enabled successful recapitulation of key features of …

A fully automated high-throughput workflow for 3D-based chemical screening in human midbrain organoids

H Renner, M Grabos, KJ Becker, TE Kagermeier, J Wu… - elife, 2020 - elifesciences.org
Three-dimensional (3D) culture systems have fueled hopes to bring about the next
generation of more physiologically relevant high-throughput screens (HTS). However …

The effect of thiol structure on allyl sulfide photodegradable hydrogels and their application as a degradable scaffold for organoid passaging

FM Yavitt, TE Brown, EA Hushka, ME Brown… - Advanced …, 2020 - Wiley Online Library
Intestinal organoids are useful in vitro models for basic and translational studies aimed at
understanding and treating disease. However, their routine culture relies on animal‐derived …

Advancing drug discovery for neurological disorders using iPSC-derived neural organoids

G Costamagna, GP Comi, S Corti - International Journal of Molecular …, 2021 - mdpi.com
In the last decade, different research groups in the academic setting have developed
induced pluripotent stem cell-based protocols to generate three-dimensional, multicellular …

Intestinal models for personalized medicine: from conventional models to microfluidic primary intestine-on-a-chip

XG Li, M Chen, S Zhao, X Wang - Stem Cell Reviews and Reports, 2021 - Springer
Intestinal dysfunction is frequently driven by abnormalities of specific genes, microbiota, or
microenvironmental factors, which usually differ across individuals, as do intestinal …

Research progress, challenges, and breakthroughs of organoids as disease models

Y Huang, Z Huang, Z Tang, Y Chen… - Frontiers in Cell and …, 2021 - frontiersin.org
Traditional cell lines and xenograft models have been widely recognized and used in
research. As a new research model, organoids have made significant progress and …

Intestinal organoids: A new paradigm for engineering intestinal epithelium in vitro

S Rahmani, NM Breyner, HM Su, EF Verdu, TF Didar - Biomaterials, 2019 - Elsevier
In recent years, the advent of intestinal organoid culture systems has revolutionized in vitro
studies of the small intestine epithelium. Intestinal organoids are derived from self …

Human microphysiological models of intestinal tissue and gut microbiome

SN Steinway, J Saleh, BK Koo, D Delacour… - … in bioengineering and …, 2020 - frontiersin.org
The gastrointestinal (GI) tract is a complex system responsible for nutrient absorption,
digestion, secretion, and elimination of waste products that also hosts immune surveillance …

3D printing of sacrificial thioester elastomers using digital light processing for templating 3D organoid structures in soft biomatrices

BJ Carberry, JE Hergert, FM Yavitt, JJ Hernandez… - …, 2021 - iopscience.iop.org
Biofabrication allows for the templating of structural features in materials on cellularly-
relevant size scales, enabling the generation of tissue-like structures with controlled form …