An updated evolutionary classification of CRISPR–Cas systems
KS Makarova, YI Wolf, OS Alkhnbashi… - Nature reviews …, 2015 - nature.com
The evolution of CRISPR–cas loci, which encode adaptive immune systems in archaea and
bacteria, involves rapid changes, in particular numerous rearrangements of the locus …
bacteria, involves rapid changes, in particular numerous rearrangements of the locus …
Diversity, classification and evolution of CRISPR-Cas systems
Highlights•Two classes of CRISPR-Cas systems differ by the architectures of effector
modules.•Effectors of Class 2 CRISPR-Cas systems are large, multidomain proteins.•Effector …
modules.•Effectors of Class 2 CRISPR-Cas systems are large, multidomain proteins.•Effector …
Multiplexed and portable nucleic acid detection platform with Cas13, Cas12a, and Csm6
Rapid detection of nucleic acids is integral for clinical diagnostics and biotechnological
applications. We recently developed a platform termed SHERLOCK (specific high-sensitivity …
applications. We recently developed a platform termed SHERLOCK (specific high-sensitivity …
C2c2 is a single-component programmable RNA-guided RNA-targeting CRISPR effector
INTRODUCTION Almost all archaea and about half of bacteria possess clustered regularly
interspaced short palindromic repeat (CRISPR)–CRISPR-associated genes (Cas) adaptive …
interspaced short palindromic repeat (CRISPR)–CRISPR-associated genes (Cas) adaptive …
The biology of CRISPR-Cas: backward and forward
F Hille, H Richter, SP Wong, M Bratovič, S Ressel… - Cell, 2018 - cell.com
In bacteria and archaea, clustered regularly interspaced short palindromic repeats
(CRISPR) and CRISPR-associated (Cas) proteins constitute an adaptive immune system …
(CRISPR) and CRISPR-associated (Cas) proteins constitute an adaptive immune system …
Diversity and evolution of class 2 CRISPR–Cas systems
Class 2 CRISPR–Cas systems are characterized by effector modules that consist of a single
multidomain protein, such as Cas9 or Cpf1. We designed a computational pipeline for the …
multidomain protein, such as Cas9 or Cpf1. We designed a computational pipeline for the …
Programmable RNA targeting with the single-protein CRISPR effector Cas7-11
A Özcan, R Krajeski, E Ioannidi, B Lee, A Gardner… - Nature, 2021 - nature.com
CRISPR–Cas interference is mediated by Cas effector nucleases that are either components
of multisubunit complexes—in class 1 CRISPR–Cas systems—or domains of a single …
of multisubunit complexes—in class 1 CRISPR–Cas systems—or domains of a single …
[HTML][HTML] Biology and applications of CRISPR systems: harnessing nature's toolbox for genome engineering
Bacteria and archaea possess a range of defense mechanisms to combat plasmids and viral
infections. Unique among these are the CRISPR-Cas (clustered regularly interspaced short …
infections. Unique among these are the CRISPR-Cas (clustered regularly interspaced short …
The CRISPR-associated DNA-cleaving enzyme Cpf1 also processes precursor CRISPR RNA
I Fonfara, H Richter, M Bratovič, A Le Rhun… - Nature, 2016 - nature.com
CRISPR–Cas systems that provide defence against mobile genetic elements in bacteria and
archaea have evolved a variety of mechanisms to target and cleave RNA or DNA. The well …
archaea have evolved a variety of mechanisms to target and cleave RNA or DNA. The well …
CRISPR-Cas immunity in prokaryotes
LA Marraffini - Nature, 2015 - nature.com
Prokaryotic organisms are threatened by a large array of viruses and have developed
numerous defence strategies. Among these, only clustered, regularly interspaced short …
numerous defence strategies. Among these, only clustered, regularly interspaced short …