Multiplexed CRISPR technologies, in which numerous gRNAs or Cas enzymes are
expressed at once, have facilitated powerful biological engineering applications, vastly …

Covering: 2011–2022 The low amounts of terpenoids produced in plants and the difficulty in
synthesizing these complex structures have stimulated the production of terpenoid …

Designing an optimal microbial cell factory often requires overexpression, knock-down, and
knock-out of multiple gene targets. Unfortunately, such rewiring of cellular metabolism is …

Microbial cell factories (MCFs) convert low-cost carbon sources into valuable compounds.
The CRISPR/Cas9 system has revolutionized MCF construction as a remarkable genome …

With developments in synthetic biology,“engineering biology” has emerged through
standardization and platformization based on hierarchical, orthogonal, and modularized …

Highlights•Isoprenoids are industrially important natural products.•Yeast are key industrial
production vehicles.•New synthetic biology approaches in recent years are reviewed.•Future …

Within five years, the CRISPR-Cas system has emerged as the dominating tool for genome
engineering, while also changing the speed and efficiency of metabolic engineering in …

RNA-guided genomic transcriptional regulation tools, namely clustered regularly
interspaced short palindromic repeats interference (CRISPRi) and CRISPR-mediated gene …

The methylotrophic yeast Pichia pastoris is firmly established as a host for the production of
recombinant proteins, frequently outperforming other heterologous hosts. Already, a …

CRISPR gene activation and inhibition (CRISPRai) has become a powerful synthetic tool for
influencing the expression of native genes for foundational studies, cellular reprograming …