▪ Abstract The maintenance of the eukaryotic genome requires precisely coordinated
replication of the entire genome each time a cell divides. To achieve this coordination …

Complex cellular events commonly depend on the activity of molecular “machines” that
efficiently couple enzymatic and regulatory functions within a multiprotein assembly. An …

Sliding clamps are ring-shaped proteins that encircle DNA and confer high processivity on
DNA polymerases. Here we report the crystal structure of the five-protein clamp loader …

▪ Abstract Chromosomal DNA replicases are multicomponent machines that have evolved
clever strategies to perform their function. Although the structure of DNA is elegant in its …

Clamp loaders ensure processive DNA replication by loading the toroidal shaped sliding
clamps onto the DNA. The sliding clamps serve as a platform for the attachment of …

The cellular pathways involved in maintaining genome stability halt cell cycle progression in
the presence of DNA damage or incomplete replication. Proteins required for this pathway …

Progress in Nucleic Acid Research Copyright 2004, Elsevier Inc. and Molecular Biology, Vol.
78 227 All rights reserved. 0079-6603/04 $35.00 modeling. Its modification by ubiquitin …

Loading of the Mcm2-7 DNA replicative helicase onto origin-proximal DNA is a critical and
tightly regulated event during the initiation of eukaryotic DNA replication. The resulting …

High accuracy (fidelity) of DNA replication is important for cells to preserve the genetic
identity and to prevent the accumulation of deleterious mutations. The error rate during DNA …

The structure of the E. coli β clamp polymerase processivity factor has been solved in
complex with primed DNA. Interestingly, the clamp directly binds the DNA duplex and also …